Internalization and trafficking to lysosomes are critical processes for both antibody-drug conjugates (ADCs) and G protein-coupled receptor (GPCR) therapeutics. For ADCs, internalization enables payload release in acidic endo-lysosomal compartments following target binding, while GPCR internalization regulates receptor availability at the cell surface, controlling desensitization and the duration of receptor-mediated signaling. Effective methods to quantify internalization in live cells are essential for both ADC development and GPCR characterization.
Revvity provides tools for studying internalization using both plate-reader based assays and high-content imaging. These approaches are complementary:
pHSense™ probes are plate reader-compatible reagents with time-resolved fluorescence (TRF) readout. These probes empower researchers with a scalable, sensitive, and easy-to-implement solution.
PhenoVue™ reagents for internalization studies facilitate real-time visualization of antibody trafficking into acidic cellular lysosomes with clarity and reliability.
For research use only. Not for use in diagnostic procedures.
