Solutions to support hybrid capture-based targeted sequencing
Hybrid capture-based targeted sequencing depends on more than the capture step itself. Input quality, library quality, blocker selection, workflow controls, and automation can all influence enrichment performance, sequencing efficiency, and run-to-run consistency. Explore solutions that support upstream preparation, quality assessment, target enrichment, performance monitoring, and workflow scale-up.
Sample preparation
Consistent sample preparation helps reduce variability before nucleic acid extraction and downstream targeted sequencing. Homogenization solutions can support reproducible processing of tissue and other sample types, helping prepare samples for efficient DNA or cfDNA extraction.
Nucleic acid extraction
High-quality input material is critical for hybrid capture-based targeted sequencing workflows. Automated nucleic acid purification solutions can help support consistent DNA or cfDNA recovery, reduce manual handling, and improve scalability across sample types and throughput needs.
Sample and library quality control
Assessing nucleic acid and library quality before sequencing helps identify issues that can affect enrichment performance, sequencing efficiency, and data quality. Microfluidic QC solutions can support sizing, quantification, and quality assessment for genomic DNA, cfDNA, FFPE-derived DNA, and prepared libraries.
Hybrid capture blockers
Hybrid capture blockers help reduce unwanted adapter interactions during hybridization-based target enrichment. NEXTFLEX Universal Blockers are designed to simplify blocker selection across compatible library configurations, including workflows with a range of barcode or UMI lengths.
Reference standards
Reference standards can support assay development, workflow verification, performance monitoring, and troubleshooting for targeted sequencing workflows. Mimix™ standards help provide defined material for evaluating assay performance and monitoring consistency across runs, operators, instruments, or workflow changes.
Automation
Automated liquid handling can help improve consistency and scalability in targeted sequencing workflows. Automation solutions can reduce hands-on time, limit manual variability, and support higher-throughput implementation of extraction, library preparation, QC workflows, or other workflow steps.
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FAQs
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What is hybridization-based target capture?
Hybridization-based target capture is a targeted sequencing approach that uses complementary probes to enrich selected genomic regions before sequencing. This allows researchers to focus sequencing capacity on regions of interest, helping support deeper coverage of defined targets compared with broader sequencing approaches.
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How is hybrid capture different from amplicon sequencing?
Hybrid capture uses probes to enrich selected regions from prepared sequencing libraries, while amplicon sequencing uses PCR primers to amplify specific targets before sequencing. Hybrid capture is often used for larger or more complex panels, while amplicon sequencing may be used for smaller, highly defined target regions.
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When are hybrid capture blockers used?
Hybrid capture blockers are used during the hybridization step of hybridization-based target enrichment. They are designed to bind adapter sequences and help reduce unwanted adapter-adapter interactions that can compete with probe-target hybridization or contribute to off-target sequencing.
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Why does library quality matter in hybrid capture-based targeted sequencing?
Library quality can influence how efficiently target regions are enriched and sequenced. Poor library yield, broad size distributions, adapter dimers, or degraded input material can affect capture performance, sequencing efficiency, and data quality.
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Why are reference standards useful for targeted sequencing workflows?
Reference standards provide defined material that can be used to support assay development, workflow verification, performance monitoring, and troubleshooting. They can help evaluate whether a targeted sequencing workflow is performing consistently across runs, operators, instruments, or workflow changes.
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Can hybrid capture-based targeted sequencing workflows be automated?
Yes. Many steps in targeted sequencing workflows can be automated, including nucleic acid extraction, liquid handling, library preparation, target enrichment setup, and QC preparation. Automation can help reduce hands-on time, limit manual variability, and support higher-throughput implementation.
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