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NEXTFLEX Universal Blockers

The NEXTFLEX^® Universal Blockers are short DNA oligos that block non-specific hybridization between adapter sequences, enhancing specificity and reducing sequencing costs. They are compatible with all NEXTFLEX barcodes and all other ligation and tagmentation based workflows for Illumina® and Element® sequencing platforms. Also known as adapter-blocking oligos or universal U-Block mixes, Universal Blockers prevent index-to-index cross-hybridization in TruSeq^®, Nextera™ and Element^® libraries.

View product information ビュー related reagents

Feature	Specification
Automation Compatible	No
Product Group	Targeted Sequencing

View product information ビュー related reagents

Product variants

Unit Size: 8 rxns

Part #:

NOVA-5143231

Unit Size: 48 rxns

Part #:

NOVA-5143232

Unit Size: 96 rxns

Part #:

NOVA-5143233

For research use only. Not for use in diagnostic procedures.

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Product information

Overview

Simplify and reduce the cost of hybridization-based target capture with the NEXTFLEX universal blockers for NGS

Effective in blocking without being affected by index length or the presence of a unique molecular index (UMI)
Compatible with all NEXTFLEX barcodes and all other ligation and tagmentation based workflows for Illumina and Element sequencing platforms
Simplifies and reduces the cost of target capture without sacrificing performance.

Additional product information

Improve the number of on-target reads

How adapter-blocking oligos increase on-target reads

During target enrichment, adapters can interact with the complementary adapter sequence strand of another library molecule. The NEXTFLEX Universal Blockers are designed to prevent this concatemerization of library molecules. By blocking this complementary adapter sequence interaction, the number of on-target reads improves dramatically and the depth of enrichment increases.

One set of blockers for all applications

The NEXTFLEX Universal Blockers are designed for use with indexed adapters for Illumina sequencing platforms. They are effective in blocking single-indexed or dual-indexed libraries, without being affected by index length or the presence of a unique molecular index (UMI).

Figure 1. NEXTFLEX Universal Blockers (UB) work from 0-19 bp unblocked regions (barcodes and/or UMIs). Competitor A1- and A-3 are two separate products from Competitor A.

Figure 2. NEXTFLEX Universal Blockers can block Illumina TruSeq™ or Nextera™ -style libraries. Competitor A1- and A-2 are two separate products from Competitor A.

The NEXTFLEX Universal Blockers are compatible with ligation-based workflows, such as NEXTFLEX or Illumina TruSeq library prep kits, and with any libraries prepared with NEXTFLEX Dual-Index Barcodes or NEXTFLEX Unique Dual Index Barcodes.

They are also compatible with tagmentation-based workflows, such as Illumina Nextera library prep kits.

Specifications

Other specifications

Automation Compatible	No
Product Group	Targeted Sequencing
Shipping Conditions	Shipped in Dry Ice
Unit Size	8 rxns

Citations

Tian, Yijun, et al. "Identify Regulatory eQTLs by Multiome Sequencing in Prostate Single Cells." bioRxiv, 21 June 2024, doi:10.1101/2024.06.19.599704. https://pmc.ncbi.nlm.nih.gov/articles/PMC11213234/

FAQs

What are universal blockers?

Universal blockers are short DNA oligos that bind to adapter sequences during hybridization to stop libraries cross-hybridizing.
Do universal blockers work with 6 bp and 10 bp dual indexes?

Yes - NEXTFLEX Universal Blockers cover 0–19 bp barcode or UMI lengths in a single mix.
Can I use universal blockers with Twist or IDT libraries?

Yes, they block any TruSeq-compatible adapter sequence, including Twist and IDT xGen libraries.
What concentration should I use the NEXTFLEX Universal blockers at?

Follow the supplied protocol
How should I store the universal blockers?

−20°C; avoid more than five freeze-thaw cycles for the best performance.