Powering scientific intelligence.

Opera Phenix OptIQハイコンテントスクリーニングシステムは、最難度のハイコンテントアプリケーションに対応します。

• 2カメラまたは4カメラ構成により、同時イメージングで撮像の速度を向上します。
• Synchrony Optics™は、マイクロレンズ強化型スピニングディスクで、厚みのある3Dサンプルに最適化されたピンホール間隔になっています。
• 隣接するスペクトルチャネルを異なる2点から励起するDual-view Excitationで、クロストークを最小限に抑えます。
• 高開口数水浸対物レンズにより、厚みのあるサンプルでもより多くの光を捉え、高解像度の画像取得が可能です。
• 最大100 fpsの高速フレームレートと、オプションのピペッターにより、細胞の高速な応答を捉えることができます。

Operetta CLS システムは、Operetta プラットフォームで培われた信頼性の高い、直感的で強力なデータ解析機能に、高速性と高感度を組み合わせたシステムです。新しいOperetta CLSは、ハイコンテントアナリシスに求められるあらゆる要件を提供します。さらに、Operetta CLSは、HCSワークフローの一部です。レビティは、HCSシステムや蛍光色素、マイクロプレートをはじめ、自動化やインフォマティクスまで、あらゆるソリューションを提供します。すべてを、信頼できる一社からご提供します。さらに、習得しやすく使いやすい Harmony ハイコンテントイメージングおよび解析ソフトウェアと組み合わせることで、研究者自身による解析が可能となり、日常的な解析から複雑な解析まで、幅広いアプリケーションをすぐに実施できます。

Designed for biologists, Harmony’s workflow-based interface makes the whole process of high-content analysis straightforward, even for new users with little microscopy or programming knowledge.

One high-content analysis software for all your applications

• Phenotypic Screening
  • Get robust phenotypic fingerprints based on intensity, morphology, texture and fluorescence distribution parameters
  • Easily train the software to recognize relevant phenotypes
  • Quantify even subtle phenotypic differences
• Live Cell Imaging
  • Follow phenotypic changes over time
  • Accurately quantify label-free live cell images
  • Track individual cells over time or analyze wound-healing assays
  • Analyze cell signaling dynamics using e.g. FRET based assays
  • Perform fast response assays such as calcium flux or cardiomyocyte beat rate with imaging frame rate of up to 100fps.
  • Set-up "dispense and read" assays on the Opera Phenix Plus system with liquid handling module
  • Enhance your live-cell assay capabilities through improved time-series flexibility
• 3D Cell Models
  • Speed up 3D image acquisition by targeted imaging of microtissues
  • Better understand your cell models by exploring them in the new 3D viewer and the XYZ viewer
  • Measure morphologies and volumes in 3D, count nuclei within spheroids
  • Enhance Maximum Intensity Projection Analysis using PlaneMap
  • One software for image acquisition, 3D visualization and analysis
• Rare Cell Phenotypes
  • Quickly capture your rare cell phenotypes with PreciScan™
  • Pre-screen at low resolution and acquire objects of interest at high resolution
  • Shorten your image acquisition times
  • Reduce your data volume
• Routine Assays
  • Utilize more than 30 ready-made-solutions for standard assays
  • Ensure repeatability by running established protocols
• Optional module
  • Phenologic.AI offers an efficient and reliable method for identifying cells and cellular nuclei within fluorescent and brightfield images by harnessing the power of pre-trained deep neural networks (DNNs).

  One high-content analysis software for all users

  • New Users
    • Get quantification for your cell images
    • Easy to learn – even for users with little microscopy or programming knowledge
    • Get started with 30 ready-made solutions
    • Logical workflow for easy control of all aspects of image acquisition and analysis
    • Simple building blocks to assemble image analysis sequences (step-by-step)
  • Expert Users
    • Quantify difficult phenotypes with advanced texture and morphology readouts
    • Utilize advanced building blocks to enable e.g. ratiometric FRET imaging
    • Create “stitched” global images from multiple fields of view
    • Examine samples at multiple scales – regions found in the global image can be used for analysis in the original images
  • For Lab Managers
    • Keep up with increasing research needs – analyze larger sample sizes
    • Quickly train new users to set up the instrument and analyze their images
    • Find images, metadata and results quickly via the integrated sortable database
    • View and analyze data from any computer with installation of Harmony software
    • Get application support by image analysis experts

The Phenologic.AI module represents a significant advancement in the field of cellular imaging. By harnessing the power of pretrained deep neural networks (DNNs), it offers an efficient and reliable method for identifying cells and cellular nuclei within fluorescent and brightfield images.

Phenologic.AI introduces a Find Nuclei AI building block to Harmony and Image Artist software which simplifies the detection of cellular nuclei in brightfield images - traditionally a challenging task without the use of nuclear staining. This innovation saves time by reducing the need for parameter fine-tuning and also allows the analysis of cell images to be conducted swiftly and with precision. The ability to reliably locate cellular nuclei without staining is achieved through the advanced Phenologic.AI algorithms.

Trained on a variety of diverse cell lines, Phenologic.AI is a deep neural network model offering robust identification of cellular structures, such as nuclei, and providing reliable phase contrast imaging to delineate the cytoplasmic regions of individual cells.

Phenologic.AI benefits from the use of Graphics Processing Units (GPUs), which are the computing system of choice for image analysis and significantly enhance the processing capabilities of AI-driven software modules allowing for rapid and precise analysis that is critical in biological research settings. Phenologic.AI also supports Central Processing Unit (CPU) execution.

Phenologic.AI streamlines cell imaging workflows and opens up new possibilities for understanding cellular behaviors and disease mechanisms.

Phenologic.AI is available as a software module for Harmony High Content Imaging and Analysis Software and Image Artist Image Data Management and Analysis System.

Next generation image analysis and management

Image Artist has powerful capabilities for processing, analyzing, storing, and sharing all your high-content screening and cell imaging data – so you can significantly cut your time to results.

• Fast image data processing and image analysis even for complex assays powered by high performance computing
• Easy-to-use assay building blocks with integrated Artificial Intelligence (AI) that make advanced image analysis
  straightforward – now with improved segmentation and analysis capabilities for 3D cell applications
• A central location to store all your image data with associated instrument metadata for an enduring picture of
  experiments
• Compatible with all major high-content screening and cell imaging systems
• Multi-user solution that can support your entire lab without compromising performance
• Scalable data storage to expand with your labs needs over time
• Seamless integration with Revvity Opera Phenix OptIQ™ and Operetta CLS™ high-content screening systems operated
  by Harmony™ software, as well as Signals One
  for profiling image data, hit selection, and more
• NEW! The latest version is compatible with data from a broader range of systems including the Celigo™ image
  cytometer
• NEW! Wider range of on-premise and cloud options, with AWS S3 support. Now featuring flexible tier-based storage
  options, and Intelligent-Tiering for automatic cost-savings.
• NEW! Phenologic.AI is an optional module that provides an efficient and reliable method for identifying cells
  and cellular nuclei within fluorescent and brightfield images by harnessing the power of pre-trained deep neural
  networks (DNNs).

It is also part of our complete workflow solution for high-content screening that includes PhenoVue™ cellular imaging reagents, PhenoPlate™ imaging microplates, HCS instruments, image cytometers, software, and automation.

For all your high-content screening and cellular imaging data

High-content screening and cellular imaging experiments generate huge amounts of image data. With the latest applications such as phenotypic screening, cell painting, and 3D imaging which use more complex,
physiologically-relevant disease models, the volume of data continues to grow.

To maximize all this valuable data, you need a powerful solution for image processing, management and storage, and for sharing data with colleagues.

Image Artist allows you to bring together cell imaging data from a wide range of sources on a single software platform so that you can store, share, analyze, and re-analyze it seamlessly.

Not only does it make managing image data easier for research scientists but also for your IT team. With cloud deployment options (such as AWS S3), your IT team have just one platform and installation to manage and store research image data which can easily be scaled. They can also have peace of mind with enhanced security for cloud deployments. What's more, with AWS S3, you can choose from a range of storage classes based on your cost and data access requirements, and optional Intelligent-Tiering allows automatic transfer of data to more cost-effective tiers, based on access frequency.

Image analysis designed for biologists

Whether you are performing phenotypic screening, cell painting, 3D imaging, live cell imaging, or more routine assays, Image Artist is designed to make it easy for biologists to perform sophisticated image analysis without any coding experience.

The software platform’s image analysis building blocks encapsulate many years of knowledge and expertise in cellular imaging and analysis - so that you can focus on the biology. By simply adding together building blocks such as “Find Nuclei” and “Calculate Cell Painting Properties”, users can quickly create image analysis protocols in just a few simple steps.

With 3D cell models bridging the gap between 2D cell cultures and in vivo animal models, Image
Artist has been further optimized for 3D cell applications, with improved segmentation and analysis capabilities – so you can generate more accurate results than ever.

Built-in Artificial Intelligence (AI) and machine learning technologies allow users to train the software to develop image analysis algorithms. While other systems may require an image analysis expert to create an algorithm, Image Artist uses proprietary machine-learning technology to make it easy for you to do it on your own. Using a learn-by-example approach, in just a few clicks segmented images can be classified quickly and easily.

This is all powered by high performance computing, so you can get your answers faster.

• Get started without extensive training
• Measure complex and subtle phenotypic responses
• Compare multiple samples, plates, or batches to quality check your results
• Kinetic measurement capabilities to track cells, reveal changes in cell properties, and provide information on
  cell movement
• Use with Signals One to perform screening data analysis and validation, QC analyses,
  calculate reliable normalization, multivariate hit stratification, dose response curves, and drug response
  profiling

Revvity's PhenoVue™ suite of cellular imaging reagents – including cell painting kits, organelle and cell compartment stains