RNA sequencing library prep kits and transcriptomics solutions

RNA sequencing enables comprehensive analysis of gene expression and RNA biology across coding and non-coding transcripts. Revvity supports transcriptomics workflows spanning whole transcriptome profiling, screening-scale gene expression studies, and depletion strategies that improve detection of biologically relevant signals.

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Selection guide

Item	Revvity solution
Whole transcriptome profiling	NEXTFLEX Rapid Directional Rapid 2.0 RNA UDI 2.0 Barcodes PolyA RNA Depletion
Screening-scale transcriptomics	DRUG-Seq and Total DRUG-seq cat page

Item

Revvity solution

Whole transcriptome profiling

NEXTFLEX Rapid Directional Rapid 2.0

RNA UDI 2.0 Barcodes

PolyA

RNA Depletion

Screening-scale transcriptomics

DRUG-Seq and Total DRUG-seq cat page

Main applications for RNA-seq:

RNA-seq supports a broad range of discovery and translational research applications, from transcript quantification to pathway analysis and high-throughput perturbation studies.

Gene expression profiling

Quantify transcript levels to characterize cellular states and biological processes.

Differential expression analysis

Identify genes and pathways altered between conditions, such as treated vs control or healthy vs diseased samples.

Screening and mechanism-of-action analysis

Link gene expression signatures to chemical or genetic perturbations to support hit prioritization, on-target assessment, off-target detection, and MoA studies.

Transcript discovery and isoform analysis

Resolve transcript structure, alternative splicing, and novel transcript features across complex samples.

Non-coding RNA profiling

Study regulatory RNAs, including long non-coding RNAs, and circular RNAs. For dedicated workflows targeting short non-coding species, explore our Small RNA Sequencing Library Prep Kits & Accessories.

For research use only. Not for use in diagnostic procedures.

RNA library prep and accessory products

Your RNA-seq hub

Everything you need for RNA sequencing, all in one place. Revvity offers a transcriptomics portfolio that includes whole transcriptome library prep, enrichment and depletion workflow components, and scalable gene expression solutions for screening applications. Explore the products below to match workflow needs, sample type, throughput, and sequencing strategy.

Whole transcriptome profiling

Revvity offers flexible, strand-specific library preparation for total RNA workflows, with accessory options for multiplexing, poly(A) enrichment and depletion, depending on sample quality, RNA class, and study goals.

Diagram of classic RNA-seq workflow with points for enrichment and depletion product use

Figure 1. This figure illustrates the classic RNA-seq workflow. The numbers in the diagram show where various enrichment & depletion based accessory products can be used in RNA-seq workflows.

Screening-scale transcriptomics

Screening-scale transcriptomics is designed for studies that require gene expression readouts across hundreds to thousands of chemical or genetic perturbations. Extraction-free workflows with early multiplexing reduce hands-on steps and cost per sample while enabling hit prioritization, mechanism-of-action analysis, and off-target assessment at scale. Explore DRUG-seq and Total DRUG-seq solutions.