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Cellaca PLX ハイスループットイメージサイトメーター

Cellaca™ PLXは細胞解析ワークフローに適したハイスループットイメージサイトメーターです。24サンプルを5分未満で細胞数カウント、濃度測定、および生存率が同時に測定できます。さらに、Cellaca PLXは表面マーカー、アポトーシスマーカー、ならびにトランスダクションおよびトランスフェクション効率を測定できます。これらすべてをラボベンチで行うことが可能です。

本製品は研究用です。診断用にはご使用いただけません。

Part #: PLX-SYS1

Imaging Modality:

蛍光・明視野

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Cellaca PLX ハイスループットイメージサイトメーター

Cellaca-PLX-with-reagents

Cellaca PLX ハイスループットイメージサイトメーター

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蛍光・明視野

Imaging Modality: 蛍光・明視野

Part #: PLX-SYS1

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Product information

Overview

Cellaca PLXイメージサイトメーターは、単純な細胞数、濃度、細胞生存率の測定から、より複雑なマルチプレックスアッセイに至るまで、多様な細胞解析に対応します。わずか15～50 µLのサンプルで実施可能です。システムの感度により、デュアル蛍光および明視野イメージングを使用して24サンプルまでを5分未満で解析可能です。さらに4チャンネルでの単一サンプルのマルチプレックスは1分で行えます。

免疫表現型解析、アポトーシス、蛍光タンパク質発現のためのマルチプレックスパネルは、Cellaca PLX低蛍光スライド、専用試薬、組み込みアッセイ、カスタマイズ可能なデータレポートを使用することで、次の工程までの時間を大幅に短縮します。Cellaca PLXは、ユーザーフレンドリーなソフトウェア、オートメーション互換性を備え、21 CFR Part 11対応です。細胞株の開発、細胞分離、遺伝子導入など、さまざまな研究用途を効率化し、お使いのワークフローにシステムを組み込むための専門的なサポートを行っています。

Cellaca PLXの主な機能と利点:

ハイスループット性能 - 細胞核と細胞のカウントを蛍光2色および明視野での撮像と解析。最大24のサンプルを5分以内で解析可能
マルチプレックス機能 - 表面マーカー、アポトーシス、および蛍光タンパク質解析用の専用試薬キットを使用し、ダウンストリーム工程までの時間を短縮（1サンプルあたり4チャンネルで1分）
少量のサンプル容量 - わずか15 µLの細胞サンプルで実施可能
直感的なワークフロー - カスタマイズされた実験やよく使用するアッセイをすぐに使えるお気に入り機能と、自動データ解析と自動レポート作成に対応
オートメーション対応 - APIを介した搬送用ロボットの接続が可能
21 CFR Part 11対応 - 監査証跡、ユーザーアクセス制御、デジタル署名にアドオンで対応

Additional product information

Cellaca PLX applications

With the Cellaca PLX platform, you can:

Quickly perform cell purity checks and viability at the bench with Cellaca PLX immunophenotyping kits which combine CD3, CD4, and CD8 surface marker detection with cell viability in a single multiplex assay.
Stain transfected cell lines with viability dyes to determine the percentage of transfected or transduced cells that are live/dead.
Perform routine viability measurements as well as apoptosis functional assays to determine cell health.

Cellaca PLX optimized assay reagents and kits

The Cellaca PLX system provides flow-like data with simple-to-use, mix, incubate, wash, and read reagent kits that provide immunophenotyping plus cell counts and viability multiplexing assays using disposable, low fluorescence consumables. Quickly perform cell counts, concentration, purity checks, and viability at the bench with Cellaca PLX immunophenotyping kits which combine CD3, CD4, and CD8 surface marker detection with cell viability in a single multiplex assay.

Available assay kits include:

Immune cell phenotyping
Apoptosis detection
High-throughput cell viability
Fluorescent protein analysis

Low auto-fluorescent consumables

Cellaca PLX consumable slides provide a safe and self-contained environment for the sample which then does not require a decontamination procedure of the instrument at the end of each run. The slide holder, which is included with the instrument, can accommodate 4 slides or 8 samples per experimental run.

Software that makes multiplexing easy

Stress-free Cellaca PLX software comes with preloaded, step-by-step templates and methodologies to streamline multiplex analysis, providing a benchtop solution for quick, reliable measurements of cell counts and concentrations, surface marker staining, viability, and apoptosis. And you get customizable results for presentations, too.

A Cellaca instrument for every analysis

The Cellaca instrument line comprises the Cellaca MX high-throughput cell counter, with brightfield and two fluorescent channels, and the Cellaca PLX image cytometry system, with brightfield and five fluorescent channels, for multichannel analysis.

	Cellaca PLX	Cellaca MX (MX-AOPI)
Channels	Brightfield, Blue, Green, Red, Far Red	Brightfield, Green, Red
Number of fluorescent channels	13 (6 per scan)	3 (2 per scan)
Excitation LED	370 nm, 475 nm, 531 nm and 628 nm	470 nm, 527nm
Emissions Filters	452 nm, 534 nm, 605 nm, 655 nm and 692 nm	534 nm, 655 nm
Commonly Used Compatible Dyes	Trypan Blue, AO/PI, Hoechst, DAPI, GFP, RFP, CMFDA, Calcein AM, 7AAD, Annexin V, PE, APC, KIRAVIA Blue 520™*	Trypan Blue, AO/PI, Calcein AM, Annexin V, Caspase 3/7
Counting Speed	Fluorescence 4-channel scan in 1 minute (per plate)	Trypan Blue - 2 seconds Fluorescence 2-channel scan- Less than 8 seconds (per well)
Volume (per well)	15 µL in slides 50 µL - 200 µL in counting plates	50 µL - 200 µL in counting plates
Size/Diameter Range	5 µm - 80 µm	5 µm - 80 µm
Concentration Range	1x10⁵ - 1x10⁷ cell/mL	1x10⁵ - 1x10⁷ cell/mL
IQ/OQ Option	Yes	Yes
21 CFR Part 11 Ready Option	Yes	Yes
Automation Compatible	Yes	Yes
Dimensions	13 in x 13 in x 16 in (33 cm x 33 cm x 41 cm)	13 in x 13 in x 16 in (33 cm x 33 cm x 41 cm)
Weight	42 lbs (19 kg)	42 lbs (19 kg)

Specifications

Physical specifications

Dimensions	13.0 in (W) x 16.0 in (D) x 13.0 in (H)
Weight	20.0 kg

Other specifications

21CFR Compatible	Yes
Automation Compatible	Yes
Brand	Revvity
Imaging Modality	蛍光・明視野
Model	Cellaca PLX ハイスループットイメージサイトメーター
Technology	マトリックスソフトウェア
Unit Size	1ユニット

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Citations

FAQs

How much volume of my sample do I need to perform a count?

The minimum loading volume of stained sample in the counting plate is 50 µl. Typically, you will stain your cell sample 1:1 with AOPI dual-fluorescent viability dye, so you can perform a cell count with as little as 25 µl of cell sample. For cell-based assays such as immunophenotyping or GFP/RFP, instead of the counting plate, you will use low-fluorescent background slides that only require 15 µl of sample. If you have a low-volume sample, you can perform a cell count in the slides as well.
Can I save the plate for later if I only have a few samples to count?

Yes, load the counting plate with as many or as few samples as you want to count. The Cellaca software will allow you to indicate which wells contain samples you wish to count and the rest of the plate can be saved for later use.
Are the counting plates compatible with multi-channel pipettes?

Yes, the counting plates are compatible with multi-channel pipettes, making it fast and easy to stain and prep your samples. The Cellaca is also compatible with liquid handlers and automation platforms and has an optional automation license add-on, making it easy to scale up your throughput.
What daily start-up or shut-down cycles do I need to run?

There are no daily start-up or shut-down cycles for the Cellaca because there are no internal fluidics and nothing that needs to get cleaned or could clog. The Cellaca uses LEDs as a light source. The only prep you need to do is turn on the instrument and start up the software, and the Cellaca is ready to count your samples right away.
How does the Cellaca count challenging or messy cell types?

The Cellaca is designed to identify live and dead nucleated cells, even in very messy samples with RBCs and debris or in irregular-shaped cell populations. By using the AOPI dual-fluorescence viability dye, only nucleated cells are counted and RBCs and debris are ignored (this method is so specific, it even works in whole blood without lysing!). The Cellaca will show you the images it captured and which cells were counted so that you can visually verify the results and trust your data.