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Cellaca PLX ハイスループットイメージサイトメーター
本製品は研究用です。診断用にはご使用いただけません。
Part #:
PLX-SYS1
Imaging Modality:
蛍光・明視野
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Cellaca PLX ハイスループットイメージサイトメーター
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Cellaca-PLX-with-reagents
Cellaca PLX ハイスループットイメージサイトメーター
Product information
Overview
Cellaca PLXイメージサイトメーターは、単純な細胞数、濃度、細胞生存率の測定から、より複雑なマルチプレックスアッセイに至るまで、多様な細胞解析に対応します。わずか15～50 µLのサンプルで実施可能です。システムの感度により、デュアル蛍光および明視野イメージングを使用して24サンプルまでを5分未満で解析可能です。さらに4チャンネルでの単一サンプルのマルチプレックスは1分で行えます。
免疫表現型解析、アポトーシス、蛍光タンパク質発現のためのマルチプレックスパネルは、Cellaca PLX低蛍光スライド、専用試薬、組み込みアッセイ、カスタマイズ可能なデータレポートを使用することで、次の工程までの時間を大幅に短縮します。Cellaca PLXは、ユーザーフレンドリーなソフトウェア、オートメーション互換性を備え、21 CFR Part 11対応です。細胞株の開発、細胞分離、遺伝子導入など、さまざまな研究用途を効率化し、お使いのワークフローにシステムを組み込むための専門的なサポートを行っています。
Cellaca PLXの主な機能と利点:
- ハイスループット性能 - 細胞核と細胞のカウントを蛍光2色および明視野での撮像と解析。最大24のサンプルを5分以内で解析可能
- マルチプレックス機能 - 表面マーカー、アポトーシス、および蛍光タンパク質解析用の専用試薬キットを使用し、ダウンストリーム工程までの時間を短縮（1サンプルあたり4チャンネルで1分）
- 少量のサンプル容量 - わずか15 µLの細胞サンプルで実施可能
- 直感的なワークフロー - カスタマイズされた実験やよく使用するアッセイをすぐに使えるお気に入り機能と、自動データ解析と自動レポート作成に対応
- オートメーション対応 - APIを介した搬送用ロボットの接続が可能
- 21 CFR Part 11対応 - 監査証跡、ユーザーアクセス制御、デジタル署名にアドオンで対応
Additional product information
Cellaca PLX applications
With the Cellaca PLX platform, you can:
- Quickly perform cell purity checks and viability at the bench with Cellaca PLX immunophenotyping kits which combine CD3, CD4, and CD8 surface marker detection with cell viability in a single multiplex assay.
- Stain transfected cell lines with viability dyes to determine the percentage of transfected or transduced cells that are live/dead.
- Perform routine viability measurements as well as apoptosis functional assays to determine cell health.
Cellaca PLX optimized assay reagents and kits
The Cellaca PLX system provides flow-like data with simple-to-use, mix, incubate, wash, and read reagent kits that provide immunophenotyping plus cell counts and viability multiplexing assays using disposable, low fluorescence consumables. Quickly perform cell counts, concentration, purity checks, and viability at the bench with Cellaca PLX immunophenotyping kits which combine CD3, CD4, and CD8 surface marker detection with cell viability in a single multiplex assay.
Available assay kits include:
- Immune cell phenotyping
- Apoptosis detection
- High-throughput cell viability
- Fluorescent protein analysis
Low auto-fluorescent consumables
Cellaca PLX consumable slides provide a safe and self-contained environment for the sample which then does not require a decontamination procedure of the instrument at the end of each run. The slide holder, which is included with the instrument, can accommodate 4 slides or 8 samples per experimental run.
Software that makes multiplexing easy
Stress-free Cellaca PLX software comes with preloaded, step-by-step templates and methodologies to streamline multiplex analysis, providing a benchtop solution for quick, reliable measurements of cell counts and concentrations, surface marker staining, viability, and apoptosis. And you get customizable results for presentations, too.
A Cellaca instrument for every analysis
The Cellaca instrument line comprises the Cellaca MX high-throughput cell counter, with brightfield and two fluorescent channels, and the Cellaca PLX image cytometry system, with brightfield and five fluorescent channels, for multichannel analysis.
| Cellaca PLX | Cellaca MX (MX-AOPI) | |
|---|---|---|
| Channels | Brightfield, Blue, Green, Red, Far Red | Brightfield, Green, Red |
| Number of fluorescent channels | 13 (6 per scan) | 3 (2 per scan) |
| Excitation LED | 370 nm, 475 nm, 531 nm and 628 nm | 470 nm, 527nm |
| Emissions Filters | 452 nm, 534 nm, 605 nm, 655 nm and 692 nm | 534 nm, 655 nm |
| Commonly Used Compatible Dyes | Trypan Blue, AO/PI, Hoechst, DAPI, GFP, RFP, CMFDA, Calcein AM, 7AAD, Annexin V, PE, APC, KIRAVIA Blue 520™* | Trypan Blue, AO/PI, Calcein AM, Annexin V, Caspase 3/7 |
| Counting Speed | Fluorescence 4-channel scan in 1 minute (per plate) |
Trypan Blue - 2 seconds Fluorescence 2-channel scan- Less than 8 seconds (per well) |
| Volume (per well) | 15 µL in slides 50 µL - 200 µL in counting plates |
50 µL - 200 µL in counting plates |
| Size/Diameter Range | 5 µm - 80 µm | 5 µm - 80 µm |
| Concentration Range | 1x105 - 1x107 cell/mL | 1x105 - 1x107 cell/mL |
| IQ/OQ Option | Yes | Yes |
| 21 CFR Part 11 Ready Option | Yes | Yes |
| Automation Compatible | Yes | Yes |
| Dimensions | 13 in x 13 in x 16 in (33 cm x 33 cm x 41 cm) |
13 in x 13 in x 16 in (33 cm x 33 cm x 41 cm) |
| Weight | 42 lbs (19 kg) | 42 lbs (19 kg) |
Specifications
| Dimensions | 13.0 in (W) x 16.0 in (D) x 13.0 in (H) |
|---|---|
| Weight |
20.0 kg
|
| 21CFR Compatible |
Yes
|
|---|---|
| Automation Compatible |
Yes
|
| Brand |
Revvity
|
| Imaging Modality |
蛍光・明視野
|
| Model |
Cellaca PLX ハイスループットイメージサイトメーター
|
| Technology |
マトリックスソフトウェア
|
| Unit Size |
1ユニット
|
Image gallery
Cellaca PLX ハイスループットイメージサイトメーター
Cellaca PLX ハイスループットイメージサイトメーター
Video gallery
Cellaca PLX ハイスループットイメージサイトメーター
Cellaca PLX ハイスループットイメージサイトメーター
Citations
FAQs
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How much volume of my sample do I need to perform a count?
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Can I save the plate for later if I only have a few samples to count?
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Are the counting plates compatible with multi-channel pipettes?
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What daily start-up or shut-down cycles do I need to run?
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How does the Cellaca count challenging or messy cell types?
Resources
Are you looking for resources, click on the resource type to explore further.
Whitepaper
Exploring factors causing differences between cell counting methods
White paper highlighting cell counting methods and the differences between them.
Whitepaper
Exploring factors impacting and improving cell counting precision
Cell counting precision is a critical parameter for determining the overall quality of cell counting results. Many factors...
Whitepaper
History of immunotherapy and CAR T-cells
One of the most promising fields in cancer research is immunotherapy – manipulating the existing immune system to specifically...
Whitepaper
Identifying and resolving the sources of hemacytometer counting error through automation
The hemacytometer is an integral part of all cell-based research, and yet sources of error inherent in its design and utilization...
Whitepaper
Overcoming the technical and economic hurdles of single-cell sequencing
This whitepaper draws on insights from a recent expert panel discussion, examining current trends, challenges and opportunities in...
Whitepaper
Practical cell counting method selection to increase the quality of results based on ISO cell counting standards
The importance of cell counting has increased significantly in the last decade due to the major advances in the fields of cell and...
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