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  • 研究・開発
  • 細胞分析
  • Cellular Imaging & Analysis
  • ハイコンテントスクリーニング装置
  • Operetta CLS ハイコンテントアナリシスシステム

Operetta CLS ハイコンテントアナリシスシステム

Operetta CLS™ ハイコンテントアナリシスシステムの導入は、日常的なアッセイや革新的なアプリケーションにおいて、新しい生物学的知見を得ることができます。ユニークな技術を備えたOperetta CLSは、表現型解析のために必要な速度、感度、解像度を備えており、さらに、シンプルながら強力なHarmonyソフトウェアによって、Operetta CLSは微細な表現型の差異も見つけ出すことができます。

本製品は研究用です。診断用にはご使用いただけません。
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Operetta CLS High-Content Analysis System
Operetta CLS High-Content Analysis System
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Operetta CLS ハイコンテントアナリシスシステム
Operetta CLS High-Content Analysis System
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Operetta CLS High-Content Analysis System
Operetta CLS High-Content Analysis System
Operetta CLS ハイコンテントアナリシスシステム
Operetta CLS High-Content Analysis System
Operetta CLS High-Content Analysis System
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Part #: HH16000020
Imaging Modality:
明視野, 共焦点, デジタル位相差, 蛍光
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High Content Screening Instruments

Your complete HCS workflow

The Operetta CLS combines speed and sensitivity with intuitive analysis. Integrated with our complete HCS workflow and Harmony software, it empowers biologists to run complex analyses independently - right out of the box.

Get superior results with our PhenoPlate™ microplates for high content screening.
Use together with our PhenoVue™ cellular imaging reagents including cell painting kits.
Improve throughput and productivity by automating your Operetta CLS system, or benefit from our automated cellular workflows and drug discovery workflows.
Export results automatically into Signals Image Artist™ Image Analysis and Management platform, so you can access, re-analyze, store, and share all your cell image data from Operetta CLS and other HCS systems.
Combine with Signals VitroVivo for powerful multivariate statistical methods and unsupervised machine learning techniques so you can identify parameters that best define distinct cellular fingerprints.

Video overview

Key features

Intelligent image acquisition

Target more accurately your object of interest for significantly reduced acquisition and analysis times, particularly valuable for 3D microtissue and rare event studies.

Machine learning

Easily create algorithms without being an image analysis expert using our PhenoLOGIC proprietary machine-learning technology.

Easily quantify cellular phenotypes in complex 3D models

Explore your cell models by visualizing them in a 3D- and an XYZ-viewer and quantify volumetric and other 3D related phenotypic readouts.

Powerfully simple analysis capabilities

Harmony high-content imaging and analysis software - is the easy-to-learn, easy-to-use software that can make you more productive - faster.

High efficiency excitation design

Direct coupling limits the light loss that is typical of light guides or fibers.

Fast frame rate imaging

Capture rapid cellular changes with an imaging frame rate up to 105 fps.

Automated water-immersion objectives

From the company with more than 20 years of experience - giving faster exposure times, increased resolution and reduced photodamage.

Confocal spinning-disk technology

Provides a gentle imaging process (especially for live-cell experiments) with efficient background rejection.

Operetta CLS applications

Fixed-cell assays

Up to eight high-power excitation sources and user-accessible emission filters enable maximum flexibility of fluorescent stains and labels, plus the system features widefield and spinning-disk fluorescent imaging.

Live-cell assays

The spinning disk confocal optics and synchronized LED illumination provides stable excitation and minimize phototoxicity and bleaching for meaningful live-cell assays. You can also choose brightfield or digital-phase contrast imaging modes.

Complex cellular models

The large-format sCMOS camera with water-immersion objectives provides sensitivity and high resolution, while advanced software helps you address the imaging and analysis challenges presented by complex cellular models.

Advanced assays

FRET is a powerful tool for investigating conformational changes and protein-protein interactions. The Operetta CLS system’s sensitive imaging and dedicated analysis tools for ratiometric imaging, facilitate robust results.

Phenotypic fingerprinting

The Operetta CLS combines high-resolution imaging with advanced software tools to help create robust phenotypic fingerprints of the subtle differences at the core of successful phenotypic assays.

Operetta CLS applications

Read how the Operetta CLS assists in university research

We have used the Operetta and Operetta CLS high-content devices from Revvity extensively to quantitate cell behavior. It is important to combine an offer of intuitive off-the shelf solutions whilst allowing further analysis tools to be developed by users.

Dr. Davide Danovi

Centre for Stem Cells & Regenerative Medicine, King's College London

Over the last few years, Operetta CLS has become increasingly important for our analysis and it provide lots of insights from a single transwell filter or from organoids in multiwell plates. Not only do you retrieve a lot of information, but you also get fantastic pictures. For example, we can visualize the interactions of SARS-CoV-2 with the multi-layered epithelia.

Univ. Prof. Dr. Doris Wilflingseder

Institute of Hygiene and Medical Microbiology, Medical University of Innsbruck

Operetta CLS configurations

Operetta CLS Quattro

Operetta CLS Quattro

Ideal for common applications that need sensitivity and resolution, with the capacity to grow if the need arises.

Operetta CLS FLEX

Operetta CLS FLEX

Offers flexibility in excitation and imaging modes for many challenging applications – and it can be upgraded to even higher performance.

Operetta CLS LIVE

Operetta CLS LIVE

Ideal for gentle yet highly sensitive live-cell imaging.

Configuration details

    Quattro FLEX LIVE
System Options Camera sCMOS sCMOS sCMOS
Number of LEDs 4 8 8
Confocal Disk º • •
Environmental Control º º •
Water Immersion º º •
Transmitted Light • • •
Robotics/Automation Compatible • • •
Imaging Modes Fluorescence Widefield Imaging • • •
Confocal Fluorescence º • •
Brightfield and Digital Phase Contrast • • •
Ratiometric FRET • • •
3D Imaging º • •
Live-Cell Imaging º º •

• Includedº Optional

Image acquisition and analysis simplified

Image acquisition and analysis simplified with Harmony software

Intuitive workflow

Harmony software offers an intuitive user interface that guides you from image acquisition to analysis and evaluation.

Templates for quick set-up

Templates allow you to set up acquisition channels and parameters efficiently.

Ready-made solutions

Choose from pre-built solutions for common image analysis tasks, simplifying your workflow.

Customizable building blocks

Create, configure, and customize your own high-content analysis applications using image analysis building blocks.

Advanced features

Harmony includes advanced analysis capabilities, such as texture and STAR morphology analysis, providing detailed descriptions of cellular morphology and robust differentiation of phenotypes.

Data management

The software automatically stores analysis results and metadata, including assay layout, instrument settings, and user-defined keywords and annotations.

The Operetta CLS high-content analysis system highlights

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3D cell models

Enrich your research

The Operetta CLS system can analyze 3D cell cultures to help generate more physiologically relevant data for better-informed decisions.

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Brochure

Operetta CLS at a glance

Discover the features and benefits of the Operetta CLS high-content analysis system in detail.

Download now
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Reagents

Ideal for the Operetta CLS

Revvity's PhenoVue suite of cellular imaging reagents complement our proven HCS instruments.

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Meet our users

Hear from scientists

Read how researchers are using our HCS products to advance their research in a variety of fields.

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Image gallery

Product specification
Primary rat neurons imaged on the Operetta CLS high-content analysis system.
"Human ovarian granulosa tumor cell line imaged on the Operetta CLS high-content analysis system. "
iPSC-derived human cortical neurons imaged on the Operetta CLS high-content analysis system.
Macrophages imaged on the Operetta CLS high-content analysis system.
Drosophila abdomen imaged on the Operetta CLS high-content analysis system.
iPSC-derived motor neurons imaged on the Operetta CLS high-content analysis system.
HPAF-II cells imaged on the Operetta CLS  high-content analysis system.
HeLa cells imaged on the Operetta CLS high-content analysis system.
Primary rat neurons imaged on the Operetta CLS high-content analysis system.

Primary rat neurons imaged on the Operetta CLS high-content analysis system

"Human ovarian granulosa tumor cell line imaged on the Operetta CLS high-content analysis system. "

Human ovarian granulosa tumor cell line imaged on the Operetta CLS high-content analysis system

iPSC-derived human cortical neurons imaged on the Operetta CLS high-content analysis system.

iPSC-derived human cortical neurons imaged on the Operetta CLS high-content analysis system

Macrophages imaged on the Operetta CLS high-content analysis system.

Macrophages imaged on the Operetta CLS high-content analysis system

Drosophila abdomen imaged on the Operetta CLS high-content analysis system.

Drosophila abdomen imaged on the Operetta CLS high-content analysis system

iPSC-derived motor neurons imaged on the Operetta CLS high-content analysis system.

iPSC-derived motor neurons imaged on the Operetta CLS high-content analysis system

HPAF-II cells imaged on the Operetta CLS  high-content analysis system.

HPAF-II cells imaged on the Operetta CLS high-content analysis system.

HeLa cells imaged on the Operetta CLS high-content analysis system.

HeLa cells imaged on the Operetta CLS high-content analysis system.

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Product information

  • Overview
  • Specifications
  • Video gallery

Overview

Operetta CLSシステムは、Operettaプラットフォームから信頼を得ているパワフルで直感的なデータ解析と、スピードと感度を兼ね備えています。新しいOperetta CLSは、ハイコンテントアナリシスに必要なすべてを備えています。さらに、Operetta CLSシステムは包括的なHCSワークフローの一部です。HCSシステムやマイクロプレートをはじめ、オートメーションやインフォマティクスまで、あらゆるソリューションを提供します。すべてを、信頼できる一社からご提供します。ユーザーフレンドリーなHarmony™ソフトウェアは、研究者自身で解析を行うことを可能し、このHarmony解析ソフトウェアとハイコンテントイメージングとの組み合わせにより、日常的な解析から応用的な解析までのすべてを実行できます。

Applications

汎用的なアッセイからより複雑なアプリケーションまで、Operetta CLSハイコンテントアナリシスシステムは多様な励起波長、高感度な光学系、そして高精度なソフトウェア機能の適切な組み合わせにより、あらゆるアプリケーションから、さまざまな生物学的発見を得ることを可能にします。

固定細胞アッセイ

  • 最大8波長の高出力励起源とユーザーがアクセス可能なエミッションフィルターにより、様々な蛍光色素や、蛍光タンパクに対応可能です。さらに、アプリケーションにより広視野蛍光イメージングまたは、スピニングディスク共焦点イメージングを選択できます。

ライブセルアッセイ

  • スピニングディスク共焦点光学系と同期された高出力LED光源により、安定した励起が得られ、光毒性と褪色を最小限にしたライブセルアッセイを実現します。明視野またはデジタルフェイズコントラストイメージングモードを選択できます。

複雑な細胞モデル

  • ラージフォーマットsCMOSカメラと水浸対物レンズの組み合わせは、高感度と高解像度を提供し、高度な画像解析ソフトウェアは、複雑な細胞モデルがもたらすイメージングと解析の課題を解決します。

アドバンスドアッセイ

  • FRETは、タンパク質間相互作用や構造変化を調べるための強力なツールです。Operetta CLSシステムの高感度イメージングと、レシオメトリックイメージング専用の解析ツールにより、確実な結果が得られます。

表現型フィンガープリンティング

  • Operetta CLSの高解像度イメージングと高度な解析ソフトウェアは、細胞表現型の僅かな差異を解析・数値化し、表現型フィンガープリント作成を実現します。

HCS ワークフローをトータルでサポート

  • ハイコンテントスクリーニング用に開発されたPhenoPlate™マイクロプレートで優れた結果を得ることができます。
  • cell paintingキットを含むPhenoVue™ cellular imaging 試薬との併用
  • Operetta CLSシステムの自動化は、スループットと生産性を向上させます。または、Revvityの提供する自動化された細胞解析ワークフローや創薬ワークフローの活用は、さらなる効率化を実現します。
  • Signals Image Artist™画像解析・管理プラットフォームに結果をエクスポートすることで、Operetta CLSおよび他のHCSシステムからのすべての細胞画像データにアクセス、再解析、保存、共有することができます。
  • Signals VitroVivoの強力な多変量解析と教師なしの機械学習機能は、、明確な細胞フィンガープリントを定義するパラメータを特定することができます。

Specifications

Dimensions 66.0 cm (W) x 98.0 cm (D) x 45.0 cm (H)
Automation Compatible
Yes
Brand
Operetta CLS
Imaging Modality
明視野
共焦点
デジタル位相差
蛍光
Unit Size
1 unit

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Operetta CLS ハイコンテントアナリシスシステム
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Citations

Resources

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eBook
Guide to live-cell imaging
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eBook
Organoids in action: advanced analysis for enhanced research relevance

Organoids - miniaturized 3D models that mimic key features of human organs - are transforming the way we study biology and disease...

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eBook
Solutions for precision medicine research

The need for better profiling is clear, and technological advances are making it possible to optimize clinical trials and...

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