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NEXTFLEX Custom Small RNA Blockers
NEXTFLEX Custom Small RNA Blockers
NEXTFLEX NGS Library Prep
| Feature | Specification |
|---|---|
| Product Group | Small RNA Accessory |
Product variant
Unit Size: 1 unit
Part #:
NOVA-51313-01
For research use only. Not for use in diagnostic procedures.
NEXTFLEX Custom Small RNA Blockers
NEXTFLEX NGS Library Prep
NEXTFLEX Custom Small RNA Blockers
Product information
Overview
NEXTFLEX Custom Small RNA Blockers let you deplete specific high-abundance small RNAs so more reads are allocated to study-relevant transcripts. Designed for use with the NEXTFLEX Small RNA-Seq Kit v4, they support flexible designs across organisms and sample types.
- Targets up to 10 user-selected sequences per project (25 – 50 nt)
- Applied at the 3' adapter-ligation step of the NEXTFLEX Small RNA-Seq Kit v4 workflow
- Depletes unwanted species such as tRNA and Y RNA fragments or overexpressed miRNAs
- Helps increase effective library complexity and read utilization in small RNA-seq
- Compatible with default tRNA/YRNA Blockers and with NEXTFLEX blood miRNA blockers
- Submit target sequences in FASTA format; ships lyophilized with use instructions
Additional product information
Maximize discovery by reducing dominant RNA species in small RNA-Seq
Small RNA sequencing profiles a wide array of RNA molecules under 200 nucleotides, including microRNA (miRNA), tRNA, Y RNA, PIWI-interacting RNA (piRNA), and more. However, in many samples, a few highly abundant RNA species, particularly tRNA fragments, Y RNA fragments, and a handful of overexpressed miRNAs dominate read counts. This skewed abundance profile reflects real biology but reduces library complexity, making it harder to detect rare yet biologically important RNAs.
Stop abundant RNAs from taking over your small RNA-seq data. NEXTFLEX Custom Small RNA Blockers selectively remove high-abundance RNAs from your libraries without affecting other sequences.
- Affordable and tailored for your project. Target specific miRNAs, tRNA fragments, rRNA fragments, or Y RNAs from any organism.
- Compatible with NEXTFLEX Small RNA-seq Kit v4.
- Reduce unwanted sequences without introducing bias in other RNAs.
- Free up sequencing capacity for the RNAs that matter most to your study.
How it works
- Blockers are sequence-specific oligonucleotides designed to hybridize up to 10 unique target sequences chosen by the user. Target sequences must have a length of 25 - 50 nt.
- Blocking occurs in the first step (Step A) of the NEXTFLEX Small RNA-Seq Kit v4 workflow.
- Hybridized RNAs cannot undergo 3' adapter ligation, preventing amplification downstream.
Get started
Schedule a consultation or contact our team to design custom blockers for your species, tissue, or experimental context. Increase the complexity, sensitivity, and value of your small RNA sequencing today.
Specifications
| Product Group |
Small RNA Accessory
|
|---|---|
| Shipping Conditions |
Shipped in Dry Ice
|
| Unit Size |
1 unit
|
FAQs
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In what format should I send the target sequences?
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How are the custom blockers shipped?
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When are the custom blockers used?
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Do the custom small RNA blockers work with t/YRNA blockers?
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Do the custom small RNA blockers work with NEXTFLEX blood miRNA blockers?
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Do you guarantee complete depletion?
Resources
Are you looking for resources, click on the resource type to explore further.
Form
NEXTFLEX custom small RNA blocker request form
This form is used to fill in the required details to design Custom Small RNA Blockers.
How can we help you?
We are here to answer your questions.
