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NEXTFLEX HT Agrigenomics Low-pass WGS Kit

The NEXTFLEX™ HT Agrigenomics Low-pass WGS kit is designed for the highly efficient conversion of DNA for Low pass whole genome sequencing WGS or Skim-seq applications, from both high- and low-quality samples from breeders, into Illumina^® and Element^® compatible libraries and can be paired with analysis solutions from CURIO.

Curio Genomics is a bioinformatics software company focused on accelerating agriculture genomics research.

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Feature	Specification
Automation Compatible	Yes

Curio Genomics is a bioinformatics software company focused on accelerating agriculture genomics research.

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Product variants

Barcodes: 1 - 24

Unit Size

24 rxns

Part #:

NOVA-5141-70

Barcodes: 1 - 384

Unit Size

384 rxns

Part #:

NOVA-5141-71

Barcodes: 385 - 768

Unit Size

384 rxns

Part #:

NOVA-5141-72

Barcodes: 769 - 1152

Unit Size

384 rxns

Part #:

NOVA-5141-73

Barcodes: 1153 - 1536

Unit Size

384 rxns

Part #:

NOVA-5141-74

For research use only. Not for use in diagnostic procedures.

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Product information

Overview

The NEXTFLEX HT Agrigenomics Low Pass Sequencing Kit is a new, streamlined workflow compatible with Illumina and Element Biosciences platforms. With a wide input range, it is suitable for all kind of plant and animal samples and can be run either manually or in automated workstation. Performance of the libraries has been tested with samples homogenized and extracted with Revvity solutions.

Robust – fast and easy protocol
Wide input range – from 1 ng to 500 ng
PCR-free compatible workflow
High throughput – up to 1,536 UDI barcodes available

Additional product information

Low-pass whole genome sequencing (LP-WGS) followed by imputation is becoming the preferred method for genotyping among plant and animal breeders. LP-WGS can capture a much broader spectrum of genetic variation compared to SNP arrays and is not limited by a fixed content, speeding up the selection process and improving the reliability of predictions. The high computational demands associated with processing and imputing LP-WGS data make it less accessible for many research programs and breeding initiatives.

Revvity and CURIO now deliver a comprehensive solution that includes reagents, automation, and analysis for your WGS data, tackling many of the main challenges encountered by breeders.

Once your sequencing data is received, CURIO generates results in minutes with just a single click. From initial research to large‐scale routine workflows, CURIO provides leading imputation analysis for both plant and animal breeders.

You can find more detailed information here on the Curio website.

Figure 1: Example of imputation analysis detail performed by CURIO on a domestic pig sample (Sus scrofa)

Flexible multiplexing options: Up to 1,536 UDI adapters

Barcodes are supplied with the NEXTFLEX HT Agrigenomics Low Pass Sequencing Kit. We offer 1,536 color-balanced NEXTFLEX UDI adapters that improve multiplexing capabilities for both low-level and high-level multiplexing needs.

Automation compatibility

The NEXTFLEX HT Agrigenomics Low-Pass WGS Kit. is compatible with multiple liquid handlers, including the Fontus™ NGS, Sciclone® G3 NGSx, Sciclone® G3 NGSx iQ™, and Zephyr® G3 NGS workstations.

Workflow

Online demonstration

You can now schedule a one-on-one 30-minute online demonstration with our experts to cover everything you need to know about the NEXTFLEX HT Agrigenomics Low-Pass WGS Kit protocol and data analysis with Curio. Please choose a timeframe that suits you best and we will provide you a Microsoft Teams personal link to join when the time comes. We look forward to speaking with you.

A streamlined approach to genotyping with Low-Pass WGS webinar

Join us on February 13, 2025, at 10:00 AM EST to discover how low-pass whole genome sequencing (LP-WGS) is revolutionizing genotyping in plant and animal breeding. Learn from experts Dr. Charlie Johnson and Dr. Marcel Brun from Texas A&M, and Dr. Pedro Echave from Revvity, along with Shawn Quinn from Curio Genomics.