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NEXTFLEX NGS Library Normalization Beads

NEXTFLEX™ NGS Library Normalization Beads enable fast, on-bead library normalization for NGS library prep. During the final cleanup, the beads bind a fixed mass of library DNA, equalizing molarity across samples so you can skip post-library qPCR or fluorometric quantitation and manual pooling. In high-throughput runs, this saves about three hours per 96-sample batch and supports consistent cluster density for balanced sequencing on any sequencing platform. Can be optimized to work with most NGS library prep workflow.

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Feature	Specification
Automation Compatible	Yes
Product Group	NGS Accessory

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Product variant

Unit Size: 96 rxns

Part #:

NOVA-NORMBEADS-96

For research use only. Not for use in therapeutic or diagnostic procedures.

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Product information

Overview

NEXTFLEX™ NGS Library Normalization Beads capture a defined amount of library DNA during final cleanup to standardize input, promoting uniform cluster formation and balanced read counts across samples.

On-bead library normalization that equalizes sample molarity.
Eliminates separate quantitation and manual pooling, reducing hands-on time and turnaround.
Saves ~3 hours per 96-sample batch in high-throughput workflows.
Optimized protocol reduces variability that can lead to over- or under-clustering.
Can be seamlessly integrated into most library preparation protocols.
Eliminates the capital expense of buying an instrument for normalization.
Easily automated protocol can further reduce hands-on time.
Not for use with PCR-free workflows.

Additional product information

On-bead normalization with NEXTFLEX Library Normalization Beads

These beads capture a defined mass of library DNA so NGS libraries exit the workflow at comparable molarity. This removes post-library qPCR or fluorometric quantitation and manual pool balancing, cutting hands-on time and consumables. Labs typically save about three hours per 96-sample batch. On-bead normalization also reduces sample-to-sample variability, helping avoid over- or under-clustering and the resequencing that can follow, with no extra quantitation reagents or instruments required.

Diagram comparing traditional NGS library normalization (quantitate, size, dilute and pool; ~ 3 hours) to NEXTFLEX on-bead normalization performed during cleanup with no added time.

Figure 1: Workflow comparison of post-library normalization vs on-bead normalization with NEXTFLEX Library Normalization Beads.

nextflex normalization beads vs xp cleanup recovered dna vs input

Figure 2: Normalization beads maintain an approximately constant recovered DNA concentration across a wide range of input DNA, demonstrating true normalization. In contrast, SPRI cleanup scales with input, increasing recovered DNA over the same range.

Specifications

Other specifications

Automation Compatible	Yes
Product Group	NGS Accessory
Shipping Conditions	Shipped Ambient
Unit Size	96 rxns

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FAQs

What do Normalization Beads do?

Different samples produce libraries with different final concentrations. Normalization beads bring libraries to a common, pool-ready concentration. This minimizes the need for individual quantification and manual dilution and supports more even read distribution across samples.
When should I add the beads to my workflow?

Use them after PCR amplification.
Which library types are compatible?

The beads work with a wide range of library types including WGS, RNA-seq, amplicon, small RNA, and cfDNA libraries, with optimization required for each workflow. Use with PCR-free libraries is not recommended. Please contact us help or suggestions when optimizing your workflow.
Are the beads platform-agnostic?

Yes. They are suitable for libraries prepared for major short-read platforms such as Illumina®, Element Biosciences™ and MGI™. Follow your sequencer’s loading guidance after pooling.
What input concentration range works best?

Proper normalization requires saturating the beads with dsDNA. A broad post-PCR range is supported but for best results, we recommended using ≥ 250 ng of dsDNA input.
What normalized output should I expect?

The workflow yields a consistent eluate across wells for direct pooling. Exact concentration depends on input range and fragment distribution.
Can I pool directly after normalization?

Yes. You can pool immediately after normalization.
Do the beads affect size distribution or library complexity?

These beads are not used for size selection. Libraries retain their fragment profile and sequence complexity when inputs are within the input recommendations.
Do I need additional reagents or special equipment?

The beads will need to be reconstituted in ethanol as described in the instructions. A standard 96-well magnetic separation device and common lab consumables are also used.