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NEXTFLEX HT Agrigenomics Low-pass WGS Kit

The NEXTFLEX™ HT Agrigenomics Low pass WGS Kit converts high- or low-quality DNA into Illumina® and Element® compatible libraries for low pass whole genome sequencing (LP WGS) and skim-seq. The workflow supports library construction in as little as 2.5 hours, with 1 to 500 ng inputs, and includes unique dual indices for multiplexing up to 1,536 samples. Pair the kit with CURIO analysis to enable genotyping by imputation and move from raw reads to VCF in minutes for plant and livestock breeding programs.

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Feature	Specification
Automation Compatible	Yes

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Product variants

Barcodes: 1 - 24

Unit Size

24 rxns

Part #:

NOVA-5141-70

Barcodes: 1 - 384

Unit Size

384 rxns

Part #:

NOVA-5141-71

Barcodes: 385 - 768

Unit Size

384 rxns

Part #:

NOVA-5141-72

Barcodes: 769 - 1152

Unit Size

384 rxns

Part #:

NOVA-5141-73

Barcodes: 1153 - 1536

Unit Size

384 rxns

Part #:

NOVA-5141-74

For research use only. Not for use in diagnostic procedures.

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Product information

Overview

Built for large agrigenomics studies, the NEXTFLEX HT Agrigenomics Low Pass WGS Kit delivers consistent libraries from DNA of variable quality and easily scales from pilot to production scale. Libraries are compatible with Illumina® and Element® platforms, and the workflow pairs with CURIO analysis to streamline genotyping by imputation for plant and animal programs.

Input range from 1 to 500 ng
Up to 1,536 UDI barcodes for high throughput studies
24 and 384 reaction formats, plate friendly for manual or automated runs
Illumina® and Element® platform compatibility with seamless handoff to CURIO analysis

Additional product information

Low pass WGS and imputation with CURIO

Low-pass whole genome sequencing (LP-WGS) and Skim-seq followed by imputation is becoming the preferred method for genotyping among plant and livestock breeders. LP-WGS can capture a much broader spectrum of genetic variation compared to SNP arrays and is not limited by a fixed content, speeding up the selection process and improving the reliability of predictions. The high computational demands associated with processing and imputing LP-WGS data make it less accessible for many research programs and breeding initiatives.

Revvity and CURIO now deliver a comprehensive solution that includes reagents, automation, and analysis for your WGS data, tackling many of the main challenges encountered by breeders.

Once your sequencing data is received, CURIO generates results in minutes with just a single click. From initial research to large‐scale routine workflows, CURIO provides leading imputation analysis for both plant and animal breeders.

You can find more detailed information here on the Curio website.

CURIO imputation results showing circular variant density and genome browser allele calls for a domestic pig (Sus scrofa) low pass WGS sample.

Figure 1: Example of imputation analysis detail performed by CURIO on a domestic pig sample (Sus scrofa)

Flexible multiplexing options: Up to 1,536 UDI adapters

Barcodes are supplied with the NEXTFLEX HT Agrigenomics Low Pass Sequencing Kit. We offer 1,536 color-balanced NEXTFLEX UDI adapters that improve multiplexing capabilities for both low-level and high-level multiplexing needs.

Automation compatibility

The NEXTFLEX HT Agrigenomics Low-Pass WGS Kit. is compatible with multiple liquid handlers, including the Fontus™ NGS, Sciclone® G3 NGSx, Sciclone® G3 NGSx iQ™, and Zephyr® G3 NGS workstations.

Workflow

Online demonstration

You can now schedule a one-on-one 30-minute online demonstration with our experts to cover everything you need to know about the NEXTFLEX HT Agrigenomics Low-Pass WGS Kit protocol and data analysis with Curio. Please choose a timeframe that suits you best and we will provide you a Microsoft Teams personal link to join when the time comes. We look forward to speaking with you.

A streamlined approach to genotyping with Low-Pass WGS webinar

Watch our on-demand webinar to discover how low-pass whole genome sequencing (LP-WGS) is revolutionizing genotyping in plant and animal breeding. Learn from experts Dr. Charlie Johnson and Dr. Marcel Brun from Texas A&M, and Dr. Pedro Echave from Revvity, along with Shawn Quinn from Curio Genomics.