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HTRF IP-One Gq Detection Kit, 1,000 Assay Points

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The HTRF™ IP-One Gq Detection Kit is an immunoassay designed for detection and quantitation of IP-One as a readout of Gq-coupled GPCR activity, in a homogeneous (no-wash steps, no separation steps) format.

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Feature	Specification
Application	Second Messenger Detection
Sample Volume	14 µL

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Product variants

Unit Size: 1,000 assay points

Part #:

62IPAPEB

Unit Size: 20,000 assay points

Part #:

62IPAPEC

Unit Size: 100,000 assay points

Part #:

62IPAPEJ

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption, and disposal requirements under European REACH regulations (EC 1907/2006).

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Product information

Overview

HTRF IP-One assays detect the accumulation of inositol monophosphate, a stable downstream metabolite of IP3 induced by activation of a phospholipase C (PLC) cascade. This kit allows direct characterization of all types of compounds acting on Gq-coupled receptors in either adherent or suspension cells.

From high-throughput screening (HTS) to structure-activity relationships (SAR), IP-One has been shown to offer superior benefits over traditional assays

How it works

Assay principle

The kit is based on a competitive format involving a specific antibody labeled with cryptate (donor) and IP1 coupled to d2 (acceptor). Native IP1 produced by cells competes with d2-labeled IP1 for binding to monoclonal anti-IP1 Tb2+ cryptate. LiCl is added to the cell stimulation buffer, causing IP1 to accumulate upon receptor activation.

Assay protocol

The IP-One assay features a streamlined protocol with just two incubation steps: Cell stimulation by the ligand or target compounds IP1 detection using HTRF reagentsThis protocol requires only a single, one-hour incubation period following cell stimulation.

Assay details

Specificity of IP-One Gq assay

IP-One ensures signal specificity, measuring only IP-1, an analyte that is solely produced by the action of the phospholipase enzyme (PLC) and mediated by GPCRs.

In contrast, calcium responses are complex in nature. Calcium release is frequently triggered by non-G protein mechanisms, including Ca2+ permeable channels, calcium pumps, calcium transporters.

Standard curve characteristics

Assay Window, sensitivity, reproducibility and specificity : IP-One makes no concession !

Specifications

Other specifications

Application	Second Messenger Detection
Brand	HTRF
Detection Modality	HTRF
Product Group	Kit
Sample Volume	14 µL
Shipping Conditions	Shipped Ambient
Target Class	GPCR
Technology	TR-FRET
Therapeutic Area	Cardiovascular Infectious Diseases Metabolism/Diabetes NASH/Fibrosis Neuroscience Oncology & Inflammation Rare Diseases
Unit Size	1,000 assay points