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Technical Note
A simple method for preparing GPCR membrane model extracts from stable cell lines for use with the HTRF GTP Gi binding assay
G-protein coupled receptors (GPCRs) are crucial transmembrane proteins involved in cellular signal transduction.
This technical note outlines a method for preparing GPCR membrane model extracts from stable cell lines, specifically for use with the HTRF GTP Gi binding assay.
Get this technical note and discover:
- Key Highlights such as the Importance of GPCRs and the advantages of using HTRF GTP Gi Binding Assay
- Detailed Method with Cell Culture Preparation, Cell Lysis, Membrane Preparation and Assay Optimization
For research use only. Not for use in diagnostic procedures.
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A simple method for preparing GPCR membrane model extracts from stable cell lines for use with the HTRF GTP Gi binding assay