| Feature | Specification |
|---|---|
| Application | タンパク質間相互作用 |
Revvity offers two versions of SureLight APC-SA: one protein binding assays, and one kinase assays. SureLight APC-SA protein binding assays has a ratio of APC:streptavidin that has been optimized to achieve best performance in protein binding applications.
LANCE® (Lanthanide chelate excite) and LANCE® Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One molecule of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and second molecule is labeled with an acceptor fluorophore [ULight™ dye, allophycocyanin (APC), etc.]. Upon excitation at 320 or 340 nm, energy can be transferred from donor Europium chelate to acceptor fluorophore if sufficiently close FRET (~10 nm). This results in emission of light at 665 nm (ULight dye and APC).
| Application |
Protein-Protein Interaction
|
|---|---|
| Automation Compatible |
Yes
|
| Brand |
LANCE SureLight
|
| Conjugates |
Streptavidin
|
| Detection Modality |
LANCE
|
| Fluorophore |
SureLight APC
|
| Product Group |
Fluorescent Reagent
|
| Shipping Conditions |
Shipped in Blue Ice
|
| Target Class |
Binding Assay
|
| Technology |
TR-FRET
|
| Unit Size |
30 mg
|
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This quick start guide introduces LANCE TR-FRET technology for studying protein-protein interactions, offering researchers a...
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