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Guide
LANCE protein:protein interaction quick start guide
This guide provides researchers with a comprehensive framework for developing and optimizing LANCE TR-FRET assays to study protein-protein interactions, particularly in Revvity Proprietary Information drug discovery contexts. It combines technical depth with practical protocols to support assay design, execution, and analysis.
Key offerings:
- Detailed explanation of TR-FRET principles and reagent configurations
- Step-by-step assay development using tagged proteins and toolbox reagents
- Optimization strategies for incubation times, reagent concentrations, and buffer conditions
- Guidance on validating assay specificity with small molecule inhibitors like Nutlin-3
For research use only. Not for use in diagnostic procedures.
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LANCE protein:protein interaction quick start guide