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PhenoVue Hu IgG4 Isotype Control Antibody - 2 x 96 wells
PhenoVue Hu IgG4 Isotype Control Antibody - 2 x 96 wells
Shipping box for Revvity reagent kits.
The PhenoVue™ human IgG4 isotype control is used as a negative control for test antibodies of the same isotype. Compatible with both live-cell and fixed-cell protocols, it allows for more accurate assessment of background or non-specific binding. When processed in parallel at matching concentrations, it facilitates the interpretation of antibody internalization and signal specificity.
| Feature | Specification |
|---|---|
| Quantity | 1 |
The PhenoVue™ human IgG4 isotype control is used as a negative control for test antibodies of the same isotype. Compatible with both live-cell and fixed-cell protocols, it allows for more accurate assessment of background or non-specific binding. When processed in parallel at matching concentrations, it facilitates the interpretation of antibody internalization and signal specificity.
Product variant
Unit Size: 50 µL
Part #:
PAHIGG4CT1
For research use only. Not for use in diagnostic procedures.
PhenoVue Hu IgG4 Isotype Control Antibody - 2 x 96 wells
Shipping box for Revvity reagent kits.
PhenoVue Hu IgG4 Isotype Control Antibody - 2 x 96 wells
Product information
Overview
The PhenoVue Human IgG4 Isotype Control is a negative control for evaluation of antibody-mediated internalization. Compatible with both live-cell and fixed-cell protocols, it is processed in parallel with test antibodies of the same isotype to distinguish specific uptake from nonspecific background. This reagent is recommended for use with the PhenoVue Human IgG Antibody Internalization Kit (Fluor 647, pH-sensitive), where it provides the reference needed to validate signal specificity. Supplied in a 2 x 96-well format, it supports high-content imaging, immunofluorescence, and microscopy applications, for antibody and ADC research.
Specifications
| Form |
Liquid
|
|---|
| Application |
High Content Imaging
Microscopy
|
|---|---|
| Assay Points |
2 x 96-well or 1 x 384-well microplates
|
| Brand |
PhenoVue™
|
| Detection Modality |
Fluorescence
|
| Product Group |
Antibody
|
| Quantity |
1
|
| Sample Type |
Live and fixed cells
|
| Shipping Conditions |
Shipped in Dry Ice
|
| Storage Conditions |
-16° C or below, protected from light
|
| Target Species |
Human
|
| Type |
Individual reagent
|
| Unit Size |
50 µL
|
Resources
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Application Note
Live-cell imaging of ADC internalization and trafficking
Internalization and trafficking to lysosomes are critical steps for the efficacy of antibody-based treatments, especially antibody...
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