| Feature | Specification |
|---|---|
| Color | Blue |
| Filter | DAPI |
| Organelle and Cell Compartment | 細胞核 |
PhenoVue Hoechst 33342 Nuclear stain (trihydrochloride form) is a cell-permeable organic molecule that binds preferentially to adenine-thymine (A-T) regions of the minor groove of DNA. When excited by ultraviolet light, Hoechst 33342 nuclear stain exhibits a strong fluorescence at 455 nm. It is commonly used to stain cells' nuclear compartment.
PhenoVue Hoechst 33342 Nuclear stain can be used to visualize cellular membranes in immunofluorescence, immunohistochemistry and flow cytometry, as well as high-content analysis and screening applications.
Typical working concentration: 2 µg/mL (3.24 µM)
Equivalent number of microplates:
See Product Information sheet for more information on fixed cell staining.
| Color |
Blue
|
|---|---|
| Concentration |
10 mg/mL (16.23 mM)
|
| Form |
Solution in dd H2O
|
| Maximum Emission Wavelength (Emmax) |
455 nm
|
| Maximum Excitation Wavelength (Exmax) |
357 nm
|
| Application |
High Content Imaging
Microscopy
|
|---|---|
| Brand |
PhenoVue™
|
| Detection Modality |
Fluorescence
|
| Filter |
DAPI
|
| Organelle and Cell Compartment |
Nucleus
|
| Quantity |
1 x 10 mg
|
| Sample Type |
Live and fixed samples
|
| Shipping Conditions |
Shipped Ambient
|
| Storage Conditions |
2-8 °C for short term (6 months) or -16 °C or below for long term (> 6 months), protected from light
|
| Type |
Individual reagent
|
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This is a product information sheet for PhenoVue Hoechst 33342 Nuclear Stain.
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