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  • ホーム
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  • 研究・開発
  • 細胞分析
  • Cellular Imaging & Analysis
  • Cellular Imaging Reagents
  • PhenoVue Fluorescent Probes for Organelles & Subcellular Compartments
  • PhenoVue Hoechst 33342 Nuclear Stain 100mg

PhenoVue Hoechst 33342 Nuclear Stain 100mg

PhenoVue Hoechst 33342 Nuclear Stain 100mg
HeLa cells stained with PhenoVue Hoechst 33342 Nuclear Stain
PhenoVue Hoechst 33342 Nuclear Stain 100mg
PhenoVue Hoechst 33342 Nuclear Stain 100mg
HeLa cells stained with PhenoVue Hoechst 33342 Nuclear Stain
PhenoVue Hoechst 33342 Nuclear Stain 100mg
HeLa cells stained with PhenoVue Hoechst 33342 Nuclear Stain
PhenoVue Hoechst 33342 Nuclear Stain 100mg
PhenoVue Hoechst 33342 Nuclear Stain 100mg
HeLa cells stained with PhenoVue Hoechst 33342 Nuclear Stain
PhenoVue Hoechst 33342 Nuclear Stain 100mg
HeLa cells stained with PhenoVue Hoechst 33342 Nuclear Stain

PhenoVue Hoechst 33342 nuclear stain is a cell-permeable organic molecule that binds to DNA.

PhenoVue Hoechst 33342 nuclear stain exhibits bright blue fluorescence and is validated for use in imaging microscopy and high-content screening applications.

Part of Revvity's portfolio of cellular imaging reagents, the PhenoVue Hoechst 33342 nuclear stain has a maximum excitation wavelength of 357 nm and a maximum emission wavelength of 540 nm.

View our extensive validation data in the Product Information Sheet within the Resources tab below.

View product information
Feature Specification
Color Blue
Filter DAPI
Organelle and Cell Compartment Nucleus

PhenoVue Hoechst 33342 nuclear stain is a cell-permeable organic molecule that binds to DNA.

PhenoVue Hoechst 33342 nuclear stain exhibits bright blue fluorescence and is validated for use in imaging microscopy and high-content screening applications.

Part of Revvity's portfolio of cellular imaging reagents, the PhenoVue Hoechst 33342 nuclear stain has a maximum excitation wavelength of 357 nm and a maximum emission wavelength of 540 nm.

View our extensive validation data in the Product Information Sheet within the Resources tab below.

View product information
Product variants
Quantity: 1 x 10 mg
Part #:
CP71
Quantity: 1 x 100 mg
Part #:
CP72
For research use only. Not for use in diagnostic procedures.
Request more information
PhenoVue Hoechst 33342 Nuclear Stain 100mg
HeLa cells stained with PhenoVue Hoechst 33342 Nuclear Stain
PhenoVue Hoechst 33342 Nuclear Stain 100mg
HeLa cells stained with PhenoVue Hoechst 33342 Nuclear Stain
PhenoVue Hoechst 33342 Nuclear Stain 100mg
PhenoVue Hoechst 33342 Nuclear Stain 100mg
HeLa cells stained with PhenoVue Hoechst 33342 Nuclear Stain
PhenoVue Hoechst 33342 Nuclear Stain 100mg
HeLa cells stained with PhenoVue Hoechst 33342 Nuclear Stain
PhenoVue Hoechst 33342 Nuclear Stain 100mg
PhenoVue Hoechst 33342 Nuclear Stain 100mg
PhenoVue Hoechst 33342 Nuclear Stain 100mg
HeLa cells stained with PhenoVue Hoechst 33342 Nuclear Stain
PhenoVue Hoechst 33342 Nuclear Stain 100mg
HeLa cells stained with PhenoVue Hoechst 33342 Nuclear Stain
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Product information

  • Overview
  • Additional product information
  • Specifications
  • Spectra viewer

Overview

PhenoVue Hoechst 33342 Nuclear stain (trihydrochloride form) is a cell-permeable organic molecule that binds preferentially to adenine-thymine (A-T) regions of the minor groove of DNA. When excited by ultraviolet light, Hoechst 33342 nuclear stain exhibits a strong fluorescence at 540nm. It is commonly used to stain cells' nuclear compartment.

PhenoVue Hoechst 33342 Nuclear stain can be used to visualize cellular membranes in immunofluorescence, immunohistochemistry and flow cytometry, as well as high-content analysis and screening applications. ​​

Additional product information

Features
Numbers of vials per unit 1
Quantity or volume per vial 100mg​ (10 mg/mL - 16.23mM)​​
Form Liquid​
Storage -20°C
Recommended working concentration 2µg/mL (3.24µM)​
Maximum excitation wavelength 357 nm
Maximum emission wavelength 540 nm
Common filter set DAPI
Live cell staining Yes
Fixed cell staining Yes. See Product Information Sheet for more information
Equivalent number of microplates 170-520 x 96-well microplates
140-520 x 384-well microplates
270-800​ x 1536-well microplates

 

Specifications

Color
Blue
Form
Solution in dd H2O
Maximum Emission Wavelength (Emmax)
455 nm
Maximum Excitation Wavelength (Exmax)
357 nm
Application
High Content Imaging
Microscopy
Brand
PhenoVue™
Detection Modality
Fluorescence
Filter
DAPI
Organelle and Cell Compartment
Nucleus
Quantity
1 x 100 mg
Sample Type
Live and fixed samples
Shipping Conditions
Shipped in Dry Ice
Storage Conditions
2-8 °C for short term (6 months) or -16 °C or below for long term (> 6 months), protected from light
Type
Individual reagent

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Resources

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eBook
Guide to live-cell imaging
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Brochure
PhenoVue Cellular Imaging Reagents Brochure
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Flyer
PhenoVue Cellular Imaging Reagents Flyer

This flyer describes Revvity's PhenoVue cellular imaging reagents.

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Product Info
PhenoVue Hoechst 33342 Nuclear Stain Product Information Sheet

This is a product information sheet for PhenoVue Hoechst 33342 Nuclear Stain.

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Flyer
PhenoVue reagents & imaging microplates product list

PhenoVue reagents & imaging microplates product list

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