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Tag-lite Dopamine D2 Receptor Frozen & Labeled Cells, 200 Assay Points
Tag-lite Dopamine D2 Receptor Frozen & Labeled Cells, 200 Assay Points
Diagram of Dopamine D2 receptor labeled with Tb (transiently expressing cells)
| Feature | Specification |
|---|---|
| Application | Receptor-Ligand Binding |
Product variant
Unit Size: 200 assay points
Part #:
C1TT1D2
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption, and disposal requirements under European REACH regulations (EC 1907/2006).
Tag-lite Dopamine D2 Receptor Frozen & Labeled Cells, 200 Assay Points
Diagram of Dopamine D2 receptor labeled with Tb (transiently expressing cells)
Tag-lite Dopamine D2 Receptor Frozen & Labeled Cells, 200 Assay Points
Product information
Overview
Dopaminergic neurons and dopamine receptors are the most numerous in brain. In the brain, dopamine receptors D1 and D2 are GPCR responsible for such crucial processes as voluntary movement, sleep, regulation of food intake, attention, learning, and working memory.
Cells expressing the D2 receptor are provided pre-labeled with Terbium, and can be used to conduct receptor binding studies on the aforementioned receptor.
How it works
Assay principle
Running your D2 receptor binding assay using Tag-lite is as easy as it can gets. Simply dispense 10 µL of labeled cells into each well, followed by 5 µL of labeled ligand and 5 µL of the compound you wish to test. Like all HTRF assays, Tag-lite assays do not require any washing steps. A diagram of the procedure to be followed is given on the right.
Saturation binding (KD)
A saturation binding assay measures total and non-specific binding for increasing concentrations of ligand under equilibrium conditions. To perform the assay, the fluorescent ligand is titrated into a solution containing a fixed amount of labeled cells and then incubated to equilibrium. The HTRF ratio obtained from this titration is the total binding.
Competitive binding (KI)
A competitive binding assay is performed to measure the dissociation constant, Ki. To perform the assay, the compound is titrated into a solution containing a fixed concentration of fluorescent ligand and a fixed amount of cells.
Assay validation
Kd and Ki validation
Bromocriptine was used as reference ligand. Results may vary from one HTRF® compatible reader to another.
Specifications
| Application |
Receptor-Ligand Binding
|
|---|---|
| Brand |
Tag-lite
|
| Detection Modality |
HTRF
|
| Product Group |
Cells
|
| Shipping Conditions |
Shipped in Dry Ice
|
| Target Class |
GPCR
|
| Technology |
TR-FRET
|
| Therapeutic Area |
Cardiovascular
Infectious Diseases
Metabolism/Diabetes
NASH/Fibrosis
Neuroscience
Oncology & Inflammation
Rare Diseases
|
| Unit Size |
200 assay points
|
Video gallery
Tag-lite Dopamine D2 Receptor Frozen & Labeled Cells, 200 Assay Points
Tag-lite Dopamine D2 Receptor Frozen & Labeled Cells, 200 Assay Points
Citations
Resources
Are you looking for resources, click on the resource type to explore further.
Technical Note
A simple method for preparing GPCR membrane model extracts from stable cell lines for use with the HTRF GTP Gi binding assay
G-protein coupled receptors (GPCRs) are crucial transmembrane proteins involved in cellular signal transduction.
This technical...
Application Note
Determination of association and dissociation rates constants using the Tag-lite platform
Challenge the limits of binding kinetics studies
This Note describes how binding kinetics studies can be enriched with a Kon, Koff ...
Guide
HTRF solutions, guide to major applications
This guide provides you an overview of HTRF applications in several therapeutic areas.
Flyer
Tag-lite: the binding receptor assay portfolio addressing the adenosine receptor family
Tag-lite™ is a cell-based, no-wash alternative to traditional radioisotope assays for studying GPCR-ligand interaction.
Adenosine...
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