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Tag-lite Adrenergic β2 Receptor Frozen & Labeled Cells, 200 Assay Points
Tag-lite Adrenergic β2 Receptor Frozen & Labeled Cells, 200 Assay Points
Generic TagLite tagged and labelled GPCR primary image
| Feature | Specification |
|---|---|
| Application | Receptor-Ligand Binding |
Product variant
Unit Size: 200 assay points
Part #:
C1TT1BETA2
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption, and disposal requirements under European REACH regulations (EC 1907/2006).
Tag-lite Adrenergic β2 Receptor Frozen & Labeled Cells, 200 Assay Points
Generic TagLite tagged and labelled GPCR primary image
Tag-lite Adrenergic β2 Receptor Frozen & Labeled Cells, 200 Assay Points
Product information
Overview
Endothelin receptors can be divided into ETA and ETB receptors also called ETA1 and ETA2. Both receptors are involved in the treatment of hypertension.
Cells expressing the BETA2 receptor are provided pre-labeled with Terbium, and can be used to conduct receptor binding studies on the aforementioned receptor.
How it works
Assay principle
Running your BETA2 receptor binding assay using Tag-lite is as easy as it can gets. Simply dispense 10 µL of labeled cells into each well, followed by 5 µL of labeled ligand and 5 µL of the compound you wish to test. Like all HTRF assays, Tag-lite assays do not require any washing steps. A diagram of the procedure to be followed is given on the right.
Saturation binding (KD)
A saturation binding assay measures total and non-specific binding for increasing concentrations of ligand under equilibrium conditions. To perform the assay, the fluorescent ligand is titrated into a solution containing a fixed amount of labeled cells and then incubated to equilibrium. The HTRF ratio obtained from this titration is the total binding.
Competitive binding (KI)
A competitive binding assay is performed to measure the dissociation constant, Ki. To perform the assay, the compound is titrated into a solution containing a fixed concentration of fluorescent ligand and a fixed amount of cells.
Assay validation
Kd and Ki validation
ICI-118551 was used as reference ligand. Results may vary from one HTRF® compatible reader to another.
Specifications
| Application |
Receptor-Ligand Binding
|
|---|---|
| Brand |
Tag-lite
|
| Detection Modality |
HTRF
|
| Product Group |
Cells
|
| Shipping Conditions |
Shipped in Dry Ice
|
| Target Class |
GPCR
|
| Technology |
TR-FRET
|
| Therapeutic Area |
Cardiovascular
Infectious Diseases
Metabolism/Diabetes
NASH/Fibrosis
Neuroscience
Oncology & Inflammation
Rare Diseases
|
| Unit Size |
200 assay points
|
Video gallery
Tag-lite Adrenergic β2 Receptor Frozen & Labeled Cells, 200 Assay Points
Tag-lite Adrenergic β2 Receptor Frozen & Labeled Cells, 200 Assay Points
Resources
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