| Feature | Specification |
|---|---|
| Application | 受容体-リガンド結合 |
Over the past few years, SNAP-Tag, CLIP-Tag, and Halo-Tag technologies combined with TR-FRET have paved the road to the development of many non-radioactive, no-wash binding assays. The method is based on transfecting cells using plasmids encoding a SNAP-Tag (or CLIP-Tag, or Halot-Tag) and subsequently labeling them with acceptors or donors. SNAP-Lumi4-Tb allows the labeling of SNAP-Tagged proteins with a terbium fluorophore.
SNAP-tag® is a small fusion tag that covalently interacts with specific substrates. SNAP-tag enables the specific and covalent labeling of any protein of interest. Cells are provided unlabeled and need to be labeled with Lumi4-Terbium prior to running a binding assay. Labeling reagents are available from the Revvity catalog in 4 different sizes. For more details refer to labeling procedure.
| Application |
Receptor-Ligand Binding
|
|---|---|
| Brand |
Tag-lite
|
| Detection Modality |
HTRF
|
| Product Group |
Fluorescent Reagent
|
| Shipping Conditions |
Shipped in Dry Ice
|
| Target Class |
GPCR
|
| Technology |
TR-FRET
|
| Therapeutic Area |
Cardiovascular
Infectious Diseases
Metabolism/Diabetes
NASH/Fibrosis
Neuroscience
Oncology & Inflammation
Rare Diseases
|
| Unit Size |
100 nmol
|
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