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Tag-lite pHaloTag-A2A Plasmid, 10 µg
Tag-lite pHaloTag-A2A Plasmid, 10 µg
Tube Tag-lite GPCR plasmid
The Tag-lite A2A Adenosine plasmid is used to transiently or stably transfect cells for the purpose of developing a A2A Adenosine receptor binding assay.
| Feature | Specification |
|---|---|
| Application | 受容体-リガンド結合 |
The Tag-lite A2A Adenosine plasmid is used to transiently or stably transfect cells for the purpose of developing a A2A Adenosine receptor binding assay.
Product variant
Unit Size: 1 each
Part #:
PHTA2A
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption, and disposal requirements under European REACH regulations (EC 1907/2006).
Tag-lite pHaloTag-A2A Plasmid, 10 µg
Tube Tag-lite GPCR plasmid
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Product information
Overview
Over past few years, SNAP/HALO-Tag technology combined with TR-FRET has paved way development of many non-radioactive, no-wash, binding assays.
method is based on transfecting cells using plasmids encoding a SNAP- or HALO-Tag and subsequently labeling m with Terbium. Revvity offers a large collection of such plasmids. All GPCR genes are cloned in an expression vector directly downstream from a CMV promoter, and are provided ready protein expression and labeling.
All information on this page pertains to Tag-lite plasmid cloned with A2A Adenosine receptor.
Tag-lite assays offer many features and applications over traditional receptor/ligand binding assays:
- Non-radioactive
- Homogeneous and filtration-free
- Ready-to-use kits and reagents for binding assays
- Does not alter the receptor pharmacology
- Peer-reviewed, validated technology
- Thorough kinetic studies with true Kd and Ki values, association (Kon) and dissociation (Koff) rate constants
- Screening and profiling of biologics and large molecules
Specifications
| Application |
Receptor-Ligand Binding
|
|---|---|
| Automation Compatible |
Yes
|
| Brand |
Tag-lite
|
| Detection Modality |
HTRF
|
| Product Group |
Plasmids
|
| Shipping Conditions |
Shipped in Dry Ice
|
| Target Class |
GPCR
|
| Technology |
TR-FRET
|
| Unit Size |
1 each
|
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Tag-lite pHaloTag-A2A Plasmid, 10 µg
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Technical Note
A simple method for preparing GPCR membrane model extracts from stable cell lines for use with the HTRF GTP Gi binding assay
G-protein coupled receptors (GPCRs) are crucial transmembrane proteins involved in cellular signal transduction.
This technical...
Guide
HTRF solutions, guide to major applications
This guide provides you an overview of HTRF applications in several therapeutic areas.
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