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HTRF Tag-Lite Dopamine D2 Receptor Red Antagonist Ligand, 5,000 Assay Points
HTRF Tag-Lite Dopamine D2 Receptor Red Antagonist Ligand, 5,000 Assay Points
Tube Fluorescent Ligand Red cap
| Feature | Specification |
|---|---|
| Application | Receptor-Ligand Binding |
Product variant
Unit Size: 5,000 assay points
Part #:
L0002RED
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption, and disposal requirements under European REACH regulations (EC 1907/2006).
HTRF Tag-Lite Dopamine D2 Receptor Red Antagonist Ligand, 5,000 Assay Points
Tube Fluorescent Ligand Red cap
HTRF Tag-Lite Dopamine D2 Receptor Red Antagonist Ligand, 5,000 Assay Points
Product information
Overview
Tag-lite receptor binding assays use either a green or a red fluorescent ligand to function. This Dopamine D2 receptor red antagonist is a spiperone derivative labeled with a red emitting HTRF fluorescent probe.
This ligand is best suited for use in the Dopamine D2 and Dopamine D3a receptor binding assays.
How it works
Assay principle
Running a receptor binding assay using Tag-lite is as easy as it can get. Simply dispense 10 µL of labeled cells into each well, followed by 5 µL of labeled ligand and 5 µL of the compound you wish to test. Like all HTRF assays, Tag-lite assays do not require any washing steps. A diagram of the procedure to be followed is given on the right.
Saturation binding (KD)
A saturation binding assay measures total and non-specific binding for increasing concentrations of ligand under equilibrium conditions. To perform the assay, the fluorescent ligand is titrated into a solution containing a fixed amount of labeled cells and then incubated to equilibrium. The HTRF ratio obtained from this titration is the total binding.
Competitive binding (KI)
A competitive binding assay is performed to measure the dissociation constant, Ki. To perform the assay, the compound is titrated into a solution containing a fixed concentration of fluorescent ligand and a fixed amount of cells.
Specifications
| Application |
Receptor-Ligand Binding
|
|---|---|
| Brand |
Tag-lite
|
| Detection Modality |
HTRF
|
| Product Group |
Fluorescent Reagent
|
| Shipping Conditions |
Shipped in Dry Ice
|
| Target Class |
GPCR
|
| Technology |
TR-FRET
|
| Therapeutic Area |
Metabolism/Diabetes
Neuroscience
Oncology & Inflammation
|
| Unit Size |
5,000 assay points
|
Video gallery
HTRF Tag-Lite Dopamine D2 Receptor Red Antagonist Ligand, 5,000 Assay Points
HTRF Tag-Lite Dopamine D2 Receptor Red Antagonist Ligand, 5,000 Assay Points
Resources
Are you looking for resources, click on the resource type to explore further.
Technical Note
A simple method for preparing GPCR membrane model extracts from stable cell lines for use with the HTRF GTP Gi binding assay
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This technical...
Guide
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This guide provides you an overview of HTRF applications in several therapeutic areas.
Flyer
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Tag-lite™ is a cell-based, no-wash alternative to traditional radioisotope assays for studying GPCR-ligand interaction.
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