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DELFIA Enhancement Solution, 1,000 mL

DELFIA^® Enhancement Solution is used to create a fluorescent Europium or Samarium chelate for measuring DELFIA time-resolved fluorescence (TRF) assays. This solution is required for all DELFIA TRF assays.

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Product variant

Unit Size: 1 L

Part #:

4001-0010

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption, and disposal requirements under European REACH regulations (EC 1907/2006).

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Product information

Overview

The DELFIA® Enhancement Solution is an acidic chelating detergent solution intended for use in the quantitative determination of Eu³⁺/Sm³⁺ when using a time-resolved fluoroimmunoassay. The solution dissociates Eu³⁺/Sm³⁺ from solid phase bound Eu-labeled antibodies or proteins during a time period of a few minutes to form a homogeneous and highly fluorescent micellar chelate solution. The solution allows highly sensitive Eu³⁺/Sm³⁺ measurements to be made when using the time-resolved fluorometer. The DELFIA® Enhancement Solution is also used as part of Tb³⁺/Dy³⁺ measurement together with DELFIA® Enhancer.

Because DELFIA TRF chelates are not intrinsically fluorescent, this solution (an acidic chelating detergent solution) is added to the microplate wells after a wash step to develop the fluorescence signal. This provides highly sensitive Eu and/or Sm measurements. Suitable for use in all multianalyte assays.