Cellometer™ Auto T4自動細胞カウンターは、明視野イメージングを用いて、細胞株の濃度測定およびトリパンブルー法による生存率評価を実施できます。凝集した細胞にも対応し、必要に応じてGMP/GLP対応ソフトウェアを利用できます。
Cellometer Auto T4は、明視野イメージングとパターン認識ソフトウェアを利用して、個々の細胞を高速かつ正確に識別・カウントします。細胞数、濃度、直径、および生存率(%)が自動的に計算され、報告されます。
Cellometer Auto T4セルカウンターの特長:
The Cellometer Auto T4 simultaneously calculates cell concentration and % viability for cultured cells stained with trypan blue.
Trypan blue viability is a dye exclusion method that utilizes membrane integrity to identify dead cells. The dye is unable to penetrate healthy cells, so they remain unstained. Dead cells have a compromised cell membrane that is permeable to the trypan blue dye. Dead cells are stained blue and display as dark cells in the Cellometer software with brightfield imaging.
Within 10 seconds, the Cellometer Auto T4 instrument reports:
Counted brightfield image
Live cells = green circle
Dead (stained) cells = red circle
With the Cellometer Auto T4 Cell Counter, cell morphology can be immediately viewed on-screen.
Two images with four fields of view each are captured per cell count. This is equivalent to the area of four quadrants of a hemocytometer. Counted cells are indicated on-screen for further verification that cells in the sample are being imaged and analyzed properly.
Users can confirm that:
The Cellometer distinguishes cells from cellular debris based on size, brightness, and morphology. This allows for increased accuracy with cell counts.
The Cellometer %CV (Coefficient of Variation), including sample preparation, was well under 5% for most cell lines tested. The CVs for manual counts ranged from 7% to 20%. Automated cell counters remove inter-operator variability and subjectivity from cell counts to generate more precise data.
| Cellometer Auto T4 | Hemacytometer | ||||
|---|---|---|---|---|---|
| Concentration | n | CV | Concentration | n | CV |
| 1.17 x 106 | 4 | 2.27% | 1.11 x 106 | 3 | 8.04% |
| 1.36 x 106 | 4 | 3.17% | 1.41 x 106 | 3 | 11.89% |
| 6.36 x 105 | 4 | 8.29% | 5.58 x 105 | 3 | 9.66% |
| 1.32 x 106 | 4 | 5.57% | 1.16 x 106 | 3 | 20.65% |
| 3.81 x 105 | 4 | 2.79% | 3.70 x 105 | 3 | 19.49% |
| 9.18 x 105 | 4 | 0.39% | 7.68 x 105 | 3 | 10.60% |
| Includes |
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|---|---|
| Available accessories |
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| Imaging performance |
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| Instrument specifications |
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| PC / Laptop requirements: (If purchasing Cellometer without PC laptop) |
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| Dimensions | 3.5 in (W) x 4.2 in (D) x 12.6 in (H) |
|---|---|
| Weight |
4.7 kg
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| Brand |
Revvity
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|---|---|
| Imaging Modality |
明視野
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| Model |
Cellometer Auto T4明視野セルカウンター
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| Technology |
Cellometer Software
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| Unit Size |
1 each
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Cell counting plays a crucial role in the development and manufacturing of cell and gene therapies as well as regenerative...
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