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AlphaLISA Assay Buffer (10X), 10 mL
AlphaLISA Assay Buffer (10X), 10 mL
AlphaLISA Bottle 10mL
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption, and disposal requirements under European REACH regulations (EC 1907/2006).
AlphaLISA Assay Buffer (10X), 10 mL
AlphaLISA Bottle 10mL
AlphaLISA Assay Buffer (10X), 10 mL
Product information
Overview
Several pre-formulated buffers are available for Alpha assays. These have have been developed to meet the varying needs of different Alpha assays, and one of them may be a good choice for an assay that you are developing. AlphaLISA Immunoassay Buffer (IAB) is a broadly-applicable buffer, used in most AlphaLISA immunoassays. This buffer ontains detergent and low salt, so it can be used to lyse cells in some assays. It is generally sufficient to expose the protein epitopes of membrane proteins for Alpha assay detection.
10X formulation: 250 mM HEPES, pH 7.4, 1% Casein, 10 mg/mL Dextran-500, 5% Triton X-100 and 0.5% Kathon
Specifications
| Automation Compatible |
Yes
|
|---|---|
| Brand |
AlphaLISA
|
| Detection Modality |
Alpha
|
| Product Group |
Buffer
|
| Shipping Conditions |
Shipped in Blue Ice
|
| Technology |
Alpha
|
| Unit Size |
10 mL
|
Video gallery
AlphaLISA Assay Buffer (10X), 10 mL
AlphaLISA Assay Buffer (10X), 10 mL
Citations
Resources
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Application Note
Applicability of AlphaLISA technology to a wide spectrum of complex biological samples
Alpha (Amplified Luminescent Proximity Homogeneous Assay) technology is a bead-based, no-wash alternative to traditional ELISAs...
Technical Note
Developing a high throughput AlphaLISA assay for screening activity of biologics produced by engineered probiotic microbes
Due to limitations driven by circulatory half-life and drug target bioavailability, injected biologics often require the injection...
Application Note
Screening for inhibitors of PD-1 and PD-L1 binding with AlphaLISA technology
Many tumor cells, which under normal, healthy conditions would be recognized by the body’s T cells and thereby targeted for...
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