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Application Note
Applicability of AlphaLISA technology to a wide spectrum of complex biological samples
Alpha (Amplified Luminescent Proximity Homogeneous Assay) technology is a bead-based, no-wash alternative to traditional ELISAs. Instead of detection with an HRP substrate, the Alpha assay signal is generated by the excitation of an Eu+-coated bead that has been conjugated to the detection antibody.
Alpha technology offers a simple, straight forward workflow. No wash needed!
- Add sample
- Add Acceptor bead mix; incubate 1 hr
- Add Donor beads; incubate 30 mins
- Read on Alpha-enable microplate reader
Download this application note to see how no-wash AlphaLISA™ technology provides a more-convenient alternative to ELISA for quantitation of biomarkers in complex sample types, including tissue, serum, and plasma.
For research use only. Not for use in diagnostic procedures.
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Applicability of AlphaLISA technology to a wide spectrum of complex biological samples