AlphaLISA SureFire Ultra Human Total XBP1s Detection Kit, 10,000 Assay Points

The spliced form of X-box binding protein 1 (XBP1s) is an active transcription factor that plays a vital role in the unfolded protein response (UPR). In response to endoplasmic reticulum stress and UPR, BiP relaxes its inhibitory binding to trigger IRE1. After dimerizing and auto-phosphorylating, the IRE1 receptor initiates its mRNase activity. In order to facilitate XBP-1u mRNA's translation and subsequent translocation to the nucleus, IRE1 splices it into XBP1s. Afterward, XBP-1 functions as a transcription factor. XBP1s is an important therapeutic target in studying various diseases, including cancer, diabetes, and autoimmune and inflammatory diseases.

The AlphaLISA SureFire Ultra Human Total XBP1s Detection Kit is a sandwich immunoassay for the quantitative detection of total XBP1s in cellular lysates, using Alpha Technology.

 Formats:

• The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.
• The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.
• The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.
• The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.

AlphaLISA SureFire Ultra kits are compatible with:

• Cell and tissue lysates
• Antibody modulators
• Biotherapeutic antibodies

Alpha SureFire kits can be used for:

• Cellular kinase assays
• Receptor activation studies
• Screening