The definitive guide for setting up a successful AlphaLISA™ SureFire® Ultra™ assay

Several biological processes are regulated by protein phosphorylation. It is, therefore, no surprise that the dysregulation of protein phosphorylation is implicated in a relatively large number of diseases.

AlphaLISA SureFire Ultra assays provide a robust and reliable method for quantifying a targeted phosphorylation event in cell-based experiments. This guide contains tools and data helpful for you to perform your assays using AlphaLISA SureFire Ultra:

• A detailed description of the assay and its options
• A thorough investigation of assay conditions to obtain the optimal response from the chosen modulator and cell line
• A list of optimization steps to provide a sufficient assay window and produce the strongest results possible

For research use only. Not for use in diagnostic procedures.