Extra control lysate for the AlphaLISA™ SureFire® Ultra™ Human IRAK1 Dimer assay. This control is already provided in the AlphaLISA SureFire Ultra Human IRAK1 Dimer assay kit but can also be ordered separately.
| Feature | Specification |
|---|---|
| Application | 細胞シグナル伝達 |
| Protocol Time | 2h at RT |
Extra control lysate for the AlphaLISA™ SureFire® Ultra™ Human IRAK1 Dimer assay. This control is already provided in the AlphaLISA SureFire Ultra Human IRAK1 Dimer assay kit but can also be ordered separately.
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This control lysate is intended for use with the AlphaLISA SureFire Ultra Human IRAK1 Dimer Detection Kit or for use as a control for those running orthogonal or secondary assays along with the SureFire assay. To prepare the lysate, Karpas 299 cells were prepared at 2 x 106 cells/mL and stimulated with 10 ng/ml IL-1β for 10 minutes in HBSS + 0.1% BSA. Cells were lysed with the addition of 5X Lysis Buffer.
| Application |
Cell Signaling
|
|---|---|
| Automation Compatible |
Yes
|
| Brand |
AlphaLISA SureFire Ultra
|
| Cellular or Signaling Pathway |
NFκB signaling
|
| Detection Modality |
Alpha
|
| Product Group |
Control Lysate
|
| Protocol Time |
2h at RT
|
| Shipping Conditions |
Shipped in Blue Ice
|
| Target |
IRAK1
|
| Target Class |
Phosphoproteins
|
| Target Species |
Human
|
| Technology |
Alpha
|
| Therapeutic Area |
Inflammation
|
| Unit Size |
1 vial
|
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