AlphaLISA SureFire Ultra Human Total ErbB3 (HER3) Detection Kit, 500 Assay Points

Receptor tyrosine-protein kinase erbB3, also known as HER3 (human epidermal growth factor receptor 3) is a membrane bound protein that is encoded by the ERBB3 gene. ErbB3 has a neuregulin binding domain, however, does not have an active kinase domain and can therefore bind this ligand but is unable to convey the signal into the cell through protein phosphorylation. ErbB3 forms heterodimers with other EGF receptor family members which have kinase activity. Heterodimerization leads to the activation of pathways that trigger cell proliferation or differentiation. ErbB3 overexpression is an indicator of poor patient prognosis in human breast cancer and has also been reported in other cancers such as prostate and bladder.

The AlphaLISA SureFire Ultra Human Total ErbB3 Detection Kit is a sandwich immunoassay for the quantitative detection of total ErbB3 in cellular lysates, using Alpha Technology.

Formats

• The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.
• The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.
• The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.
• The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.

AlphaLISA SureFire Ultra kits are compatible with

• Cell and tissue lysates
• Antibody modulators
• Biotherapeutic antibodies

Alpha SureFire kits can be used for

• Cellular kinase assays
• Receptor activation studies
• Screening