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Cellaca PLX High-throughput Image Cytometer
For research use only. Not for use in diagnostic procedures.
Part #:
PLX-SYS1
Imaging Modality:
Fluorescence, Brightfield
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Cellaca PLX High-throughput Image Cytometer
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Cellaca-PLX-with-reagents
Cellaca PLX High-throughput Image Cytometer
Product information
Overview
The Cellaca PLX image cytometer provides versatile cell analysis ranging from simple cell counts, concentration, and viability readouts, to more complex multiplexed assays, all using only 15-50 µL of sample. The sensitivity of the system allows for the analysis of up to 24 samples using dual fluorescence and brightfield imaging in under 5 minutes, and single sample multiplexing with four channels in 1 minute.
Multiplex panels for immunophenotyping, apoptosis, and fluorescent protein expression shorten the time to downstream processing with Cellaca PLX low fluorescence slides, dedicated reagents, built-in assays, and configurable data reports. The Cellaca PLX features user friendly software, automation compatibility, and is 21 CFR Part 11 ready. Expert support is provided to integrate the system into current workflows to streamline cell line development, cell isolation, gene delivery, and various research applications.
Key features and benefits of the Cellaca PLX:
- Elevated throughput - run up to 24 samples in under 5 minutes using dual fluorescence and brightfield imaging to count both isolated nuclei and cells
- Multiplexing capabilities - shorten time to downstream processing (one minute per sample with four channels) using our custom reagents kits for surface marker, apoptosis, and fluorescent protein analysis
- Small sample volume - as little as 15 µL of cell sample needed
- Intuitive workflows - including a favorites feature for saving customized experiments and commonly used assays with automatic data analysis and reporting
- Automation ready - robotic integration ability with via API
- 21 CFR Part 11 ready - add-on that includes audit trail, user access control, and digital signature
Additional product information
Cellaca PLX applications
With the Cellaca PLX platform, you can:
- Quickly perform cell purity checks and viability at the bench with Cellaca PLX immunophenotyping kits which combine CD3, CD4, and CD8 surface marker detection with cell viability in a single multiplex assay.
- Stain transfected cell lines with viability dyes to determine the percentage of transfected or transduced cells that are live/dead.
- Perform routine viability measurements as well as apoptosis functional assays to determine cell health.
Cellaca PLX optimized assay reagents and kits
The Cellaca PLX system provides flow-like data with simple-to-use, mix, incubate, wash, and read reagent kits that provide immunophenotyping plus cell counts and viability multiplexing assays using disposable, low fluorescence consumables. Quickly perform cell counts, concentration, purity checks, and viability at the bench with Cellaca PLX immunophenotyping kits which combine CD3, CD4, and CD8 surface marker detection with cell viability in a single multiplex assay.
Available assay kits include:
- Immune cell phenotyping
- Apoptosis detection
- High-throughput cell viability
- Fluorescent protein analysis
Low auto-fluorescent consumables
Cellaca PLX consumable slides provide a safe and self-contained environment for the sample which then does not require a decontamination procedure of the instrument at the end of each run. The slide holder, which is included with the instrument, can accommodate 4 slides or 8 samples per experimental run.
Software that makes multiplexing easy
Stress-free Cellaca PLX software comes with preloaded, step-by-step templates and methodologies to streamline multiplex analysis, providing a benchtop solution for quick, reliable measurements of cell counts and concentrations, surface marker staining, viability, and apoptosis. And you get customizable results for presentations, too.
A Cellaca instrument for every analysis
The Cellaca instrument line comprises the Cellaca MX high-throughput cell counter, with brightfield and two fluorescent channels, and the Cellaca PLX image cytometry system, with brightfield and five fluorescent channels, for multichannel analysis.
| Cellaca PLX | Cellaca MX (MX-AOPI) | |
|---|---|---|
| Channels | Brightfield, Blue, Green, Red, Far Red | Brightfield, Green, Red |
| Number of fluorescent channels | 13 (6 per scan) | 3 (2 per scan) |
| Excitation LED | 370 nm, 475 nm, 531 nm and 628 nm | 470 nm, 527nm |
| Emissions Filters | 452 nm, 534 nm, 605 nm, 655 nm and 692 nm | 534 nm, 655 nm |
| Commonly Used Compatible Dyes | Trypan Blue, AO/PI, Hoechst, DAPI, GFP, RFP, CMFDA, Calcein AM, 7AAD, Annexin V, PE, APC, KIRAVIA Blue 520™* | Trypan Blue, AO/PI, Calcein AM, Annexin V, Caspase 3/7 |
| Counting Speed | Fluorescence 4-channel scan in 1 minute (per plate) |
Trypan Blue - 2 seconds Fluorescence 2-channel scan- Less than 8 seconds (per well) |
| Volume (per well) | 15 µL in slides 50 µL - 200 µL in counting plates |
50 µL - 200 µL in counting plates |
| Size/Diameter Range | 5 µm - 80 µm | 5 µm - 80 µm |
| Concentration Range | 1x105 - 1x107 cell/mL | 1x105 - 1x107 cell/mL |
| IQ/OQ Option | Yes | Yes |
| 21 CFR Part 11 Ready Option | Yes | Yes |
| Automation Compatible | Yes | Yes |
| Dimensions | 13 in x 13 in x 16 in (33 cm x 33 cm x 41 cm) |
13 in x 13 in x 16 in (33 cm x 33 cm x 41 cm) |
| Weight | 42 lbs (19 kg) | 42 lbs (19 kg) |
Specifications
| Dimensions | 13.0 in (W) x 16.0 in (D) x 13.0 in (H) |
|---|---|
| Weight |
20.0 kg
|
| 21CFR Compatible |
Yes
|
|---|---|
| Automation Compatible |
Yes
|
| Brand |
Revvity
|
| Imaging Modality |
Fluorescence, Brightfield
|
| Model |
Cellaca PLX High-Throughput Image Cytometer
|
| Technology |
Matrix Software
|
| Unit Size |
1 unit
|
Image gallery
Cellaca PLX High-throughput Image Cytometer
Cellaca PLX High-throughput Image Cytometer
Video gallery
Cellaca PLX High-throughput Image Cytometer
Cellaca PLX High-throughput Image Cytometer
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