AAV Reference Standards

Our AAV8 reference material for gene therapy is a highly characterized, quality reference standard. It has been rigorously tested using a range of orthogonal technologies to demonstrate its reproducibility, so that you can have greater confidence in your results.

For your convenience, our AAV8 reference standard is provided at a high concentration and with detailed characterization data for verification of your methods. You’ll also benefit from our reliable stock availability.

Revvity’s AAV Reference Standards are for internal research use only under a limited use license. Not for use in diagnostic procedures. A commercial license is required for clinical or commercial applications.

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Features at a glance Technical specifications Applications

Features at a glance

With our AAV8 reference standards, you will benefit from:

High concentration
AAV particles enriched for full capsids
Genomic titer, capsid titer, full/empty and impurities measured in multiple orthogonal assays
Detailed characterization data
Support from our experienced scientific team
Reliable stock availability

Technical specifications

AAV8 wild-type full capsids
Expressing EGFP with a CMV promoter
Genomic and capsid titer at >1E12 VG/mL in 100 µL
Formulated in DPBS, 0.001% Poloxamer 188
Sterile filtered
Stored in -80 °C

Applications

Methods used for characterization:

Genomic titer:
- qPCR with ITR primers
- ddPCR with ITR primers
- ddPCR with EGFP primers
Capsid titer:
- ELISA with AAV8 antibody
- AlphaLISATM assay with AAV8 antibody
Full capsid content:
- qPCR/ELISA
- Spectrometry
- Analytical ultra centrifugation
- Transmission electron microscopy
Functional capsid:
- Fluorescence expression
Viral protein ratio:
- SDS-PAGE
Impurity testing:
- Aggregation by DLS/SLS
- Next generation sequencing
- qPCR with host-cell DNA primers
- ELISA with host-cell protein antibody
- qPCR with plasmid backbone primers
- LAL assay for endotoxin
- qPCR with mycoplasma primers
- ELISA with endonuclease antibody

Featured resources

Technical note

AAV8 reference standard material technical note

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SDS

AAV8 reference standard safety data sheet

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COA

AAV8 reference standard certificate of analysis

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FAQs

What are the sequences for the capsid and the transgene?

All sequences are provided in the Technical Note. The capsid is an AAV8 wild-type, and the transgene is CMV-EGFP-WPRE.
How was the reference material produced?

Suspension cells based on the HEK293-F cell line were transfected with lipid-based reagent. Samples were purified by affinity chromatography, iodixanol ultracentrifugation, buffer exchange, and sterile filtration.
How can the material be used?

The AAV Reference Standard Material can be used as a positive control for analytical assays, as a standard curve for protocols such as qPCR and ELISA, or as a calibrator for new method development.

AAV Reference Standards