AAV Reference Standards

Our AAV8 reference material for gene therapy is a highly characterized, quality reference standard. It has been rigorously tested using a range of orthogonal technologies to demonstrate its reproducibility, so that you can have greater confidence in your results.

For your convenience, our AAV8 reference standard is provided at a high concentration and with open access data for verification of your methods. You’ll also benefit from our reliable stock availability.

For research use only. Not for use in diagnostic procedures. A commercial license is required for any clinical or commercial application involving AAVs developed using Revvity technologies.

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Features at a glance Technical specifications Applications

Features at a glance

With our AAV8 reference standards, you will benefit from:

High concentration
AAV particles enriched for full capsids
Genomic titer, capsid titer, full/empty and impurities measured in multiple orthogonal assays
Open access data
Support from our experienced scientific team
Reliable stock availability

Technical specifications

AAV8 wild-type full capsids
Expressing EGFP with a CMV promoter
Genomic and capsid titer at >1E12 VG/mL in 100 µL
Formulated in DPBS, 0.001% Poloxamer 188
Sterile filtered
Stored in -80 °C

Applications

Methods used for characterization:

Genomic titer:
- qPCR with ITR primers
- ddPCR with ITR primers
- ddPCR with EGFP primers
Capsid titer:
- ELISA with AAV8 antibody
- AlphaLISATM assay with AAV8 antibody
Full capsid content:
- qPCR/ELISA
- Spectrometry
- Analytical ultra centrifugation
- Transmission electron microscopy
Functional capsid:
- Fluorescence expression
Viral protein ratio:
- SDS-PAGE
Impurity testing:
- Aggregation by DLS/SLS
- Next generation sequencing
- qPCR with host-cell DNA primers
- ELISA with host-cell protein antibody
- qPCR with plasmid backbone primers
- LAL assay for endotoxin
- qPCR with mycoplasma primers
- ELISA with endonuclease antibody

Featured resources

Technical note

AAV8 reference standard material technical note

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FAQs

What are the sequences for the capsid and the transgene?

All sequences are provided in the Technical Note. The capsid is an AAV8 wild-type, and the transgene is CMV-EGFP-WPRE.
How was the reference material produced?

Suspension cells based on the HEK293-F cell line were transfected with lipid-based reagent. Samples were purified by affinity chromatography, iodixanol ultracentrifugation, buffer exchange, and sterile filtration.
How can the material be used?

The AAV Reference Standard Material can be used as a positive control for analytical assays, as a standard curve for protocols such as qPCR and ELISA, or as a calibrator for new method development.

AAV Reference Standards