Typically, thousands of clones need to be measured, which is bottlenecked by traditional cell counting methods.
Transfection/transduction optimization experiment samples
- Positive control (1)
- Negative control (1)
- Test conditions (1+n)
50 μl of sample ⟶
- Size
- Total cell concentration
- Viability
- GFP or RFP expression (whole well)
- GFP or RFP expression (individual cell level)
Cellaca™ MX High-throughput Cell Counter has been utilized to provide rapid counting of CHO cells and viability measurement in less than 5 min for 24 samples. The system shows high-precision, high-speed, and high-throughput methodology. The high-throughput cell counter has demonstrated high instrument-to-instrument precision for 32 Cellaca MX in a time frame of 1 year, showing a CV of 3.6%.
[Bell et al. 2021, Characterization of a novel high-throughput, high-speed and high-precision plate-based image cytometric cell counting method, Cell and Gene Therapy Insight].
For research use only. Not for use in diagnostic procedures.