Cell line development process
The process of developing a cell line to produce a specific protein or antibody involves multiple stages, all of which can be greatly aided by the Celigo™ image cytometer.
Transfection
- Determine transfection efficiency
- Monitor stability of transfection
Stabilized outgrowth
- Monitor growth in each well
- Identify confluent wells for passaging
- Increase growth with media optimization
Monoclonality
- Verify efficiency of limiting dilution, or single cell dispensing
- Identify single cell per well
Colony outgrowth
- Confirm single colony
Robotic integration for cell line development
Whole well images: Single cell to single colony
Well with a single cell that grew into a single colony
The Celigo provides an optional robotic API which can be controlled by various automation scheduling software applications. The Celigo is ready for integration with multiple automation partners and can be coupled with robotic arms, automated incubators and liquid handlers.
The Celigo can be used through the whole process of cell line development.
- Compatible with 96-, 384- and 1536-well plates.
- Identify wells with a single colony to avoid the time-consuming and manual identification of clones by eye.
- Measures colony size using brightfield and aids the process of selecting wells for clonal expansion.
- Automate cell line generation process with the Celigo robotic integration.
Transfection & transduction optimization
Transfection efficiency
Sample 1 (top) shows moderate transfection efficiency while
sample 2 (bottom) has lower efficiency.
Quickly identify optimal parameters for high-efficiency transfection.
Determine transient and stable transfection rates and evaluate antibiotic induction using live imaging.
- Monitor transfection efficiency on the Celigo directly
- Acquire both brightfield and green fluorescence cell images
- Identify all the cells using brightfield
- Produce scatter plot for gating GFP+ cells
- Calculate % GFP+ cells automatically
- 96- or 384-well plates
For research use only. Not for use in diagnostic procedures.