- Directly image tumor spheroids in various microwell formats
- Non-invasive brightfield imaging allows the user to image the same plate over multiple days
- Perform a two-color fluorescent viability assay
Introduction
The Celigo™ image cytometer has been developed to fully automate live cell analysis of tumorspheres. This automated morphometric analysis tool significantly reduces the time and effort needed to quantify key aspects of 3D spheres including size, growth, growth tracking over time and response to chemotherapeutics.
Measure an increase in caspase 3/7 activity in HCT116 3D tumor spheroids
HCT116R2 and HCT116 spheroids were stained with Caspase 3/7 reagent and imaged. The above images present a merge of brightfield and Caspase 3/7 (green) images.
For research use only. Not for use in diagnostic procedures.