Image and quantify transwell invasion and migration assays using suspension and adherent cells
- Image transwell invasion of adherent cells without dissociation of cells from the transwell insert
- Directly count the number of invading/migrating cells
- Fluorescently label and image invading cells
Perform and quantify transwell invasion assay by direct cell count
Suspension cells located inside the insert migrate through the porous membrane toward the chemoattractant from the bottom plate. Celigo image cytometer is used to automatically count migrated cells in the bottom plate.
Image and count the number of cells in a transwell Invasion assay
Brightfield and DAPI images of transwell invasion of adherent cancer cells
Overlay of brightfield and DAPI
High-resolution brightfield images of the transwell membrane
Thumbnail pictures and cell numbers are automatically displayed upon the completion of the cell counts
Acquisition of high-resolution DAPI and brightfield images of the transwell membrane surface in a 24 well plate format took ~ 3 minutes
For research use only. Not for use in diagnostic procedures.