- A monolayer of host cells are seeded into a 6-, 12-, or 24-well plate
- Mixtures of the virus and various dilutions of the antibodies are produced and allowed to incubate for 2 hours
- After incubation, the different mixtures are then added to the host cells and incubated for another 2 hours
- The results showed a dose dependent decrease in the formation of cytolytic plaques as the antibody concentration increased
- In addition, plaque number and size information can be directly exported into Microsoft Excel for additional plotting and analysis
The Celigo™ image cytometer can directly count
cytolytic plaques in plate
Plate-view of counted cytolytic plaques treated with different antibody concentrations in a 12-well plate
|
Plaque count |
|||
|---|---|---|---|
|
67 |
19 |
4 |
2 |
|
63 |
13 |
2 |
0 |
|
Plaque size (µm2) |
|||
|---|---|---|---|
|
453017.801 |
478668.211 |
375579.65 |
58492.7559 |
|
481462.961 |
313941.745 |
382699.547 |
NaN |
Plaque counts and size measurement from the Celigo
For research use only. Not for use in diagnostic procedures.