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Cell Counting and Image Cytometry

Automated Focus Reduction Neutralization Test Using GFP-expressing RSV

Section

Modern Virology Assays

Celigo Applications

Cell Counting Method Selection

Cell Counting and Image Cytometry FAQs

Cell-based Assays for Bioprocessing

Cell-based Assays for Gene Therapy Development

Cellometer Applications

Modern Virology Assays

Sub Section

Antibody Neutralization

Antibody Binding Inhibition

Antibody Neutralization

Cytopathic Effects

Viral Titre

Topic

Automated Focus Reduction Neutralization Test Using GFP-expressing RSV

Automated Focus Reduction Neutralization Test Using GFP-expressing RSV

Automated Imaging and Detection of Cytolytic Plaques

Cytomegalovirus Neutralization Assay Using Fluorescence

Zika Virus Microneutralization Assay

Cytomegalovirus Neutralization Assay Using Brightfield

The host cells are seeded in a 24-well microplate and incubated for 100% confluence
The cells are then infected by GFP-RSV and incubated with low, medium, and high hamster sera concentrations
Finally, the plate is scanned and analyzed with the Celigo™ image cytometer to count the number of foci in each well

img-automated-focus-reduction-neutralization-test-using-gfp-expressing-rsv-1.PNG

Zoomed in bright and fluorescent images of GFP-RSV infected foci

Automated focus reduction neutralization test using GFP-expressing RSV-2

Plate-view of the 24-wells showing reduction in number of GFP foci at high concentration of serum

For research use only. Not for use in diagnostic procedures.

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