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Western Lightning ONE Femto Ultra, Chemiluminescent Substrate (250 mL)

When proteins are low and antibodies are precious, our extreme-level pre-mixed one-component chemiluminescent horseradish peroxidase (HRP) substrate with low antibody consumption is the right choice. Western Lightning™ ONE Femto Ultra can detect low-femtogram protein levels in western blots with superior signal intensity.

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Product variants

Unit Size: 25 mL

Part #:

NEL150001EA

Unit Size: 125 mL

Part #:

NEL151001EA

Unit Size: 2 x 125 mL

Part #:

NEL152001EA

For research use only. Not for use in diagnostic procedures.

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Product information

Overview

Our most sensitive substrate in our premier line of premixed chemiluminescent HRP substrates, Western Lightning ONE Femto Ultra offers low-femtogram detection with excellent signal intensity. Western Lightning ONE Femto Ultra is designed with maximum sensitivity in a single reagent format. Stored and ready-to-use at room temperature, the 12 month extended shelf life means you can buy and use Western Lightning ONE Femto Ultra with confidence. Unit size 2 x 125 mL.

Comparison of Western Lightning™ ONE Versus Major Competitors

Performance and Signal Duration

Western blotting detection of HDAC-1 in HeLa cell lysate
Exposure time is 120 secs for each time point
Images captured with ImageQuant™ LAS 4000 from GE Healthcare

Additional product information

Performance	Signal Duration
Detection Level: Mid-picogram

Two-fold dilution series of HeLa whole cell lysate from 5 μg to 0.078 μg of total protein on nitrocellulose membrane Primary antibody:Rabbit-anti Human HDAC-1 1:2000 Secondary antibody:Goat anti-rabbit IgG HRP (2mg/mL) 1:20000
Detection Level: Mid-femtogram

Two-fold dilution series of HeLa whole cell lysate from 5 μg to 0.078 μg of total protein on nitrocellulose membrane Primary antibody: Rabbit-anti Human HDAC-1 1:5000 Secondary antibody: Goat anti-rabbit IgG HRP (2mg/mL) 1:75000
Detection Level: Low-femtogram

Two-fold dilution series of HeLa whole cell lysate from 2.5 μg to 0.039 μg of total protein on nitrocellulose membrane Primary antibody: Rabbit-anti Human HDAC-1 1:10000 Secondary antibody: Goat anti-rabbit IgG HRP (2mg/mL) 1:200000

Specifications

Other specifications

Application	Western blotting
Brand	Western Lightning ONE
Detection Modality	Chemiluminescence
Sample Type	Chemiluminescent Substrate
Shipping Conditions	Shipped Ambient
Unit Size	2 x 125 mL