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Western Lightning ONE Femto Ultra, Chemiluminescent Substrate (125 mL)

When proteins are low and antibodies are precious, our extreme-level pre-mixed one-component chemiluminescent horseradish peroxidase (HRP) substrate with low antibody consumption is the right choice. Western Lightning™ ONE Femto Ultra can detect low-femtogram protein levels in western blots with superior signal intensity.

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Product variants

Unit Size: 25 mL

Part #:

NEL150001EA

Unit Size: 125 mL

Part #:

NEL151001EA

Unit Size: 2 x 125 mL

Part #:

NEL152001EA

For research use only. Not for use in diagnostic procedures.

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Product information

Overview

Our most sensitive substrate in our premier line of premixed chemiluminescent HRP substrates, Western Lightning ONE Femto Ultra offers low-femtogram detection with excellent signal intensity. Western Lightning ONE Femto Ultra is designed with maximum sensitivity in a single reagent format. Stored and ready-to-use at room temperature, the 12 month extended shelf life means you can buy and use Western Lightning ONE Femto Ultra with confidence. Available in 125 mL size.

Comparison of Western Lightning™ ONE Versus Major Competitors

Performance and Signal Duration

Western blotting detection of HDAC-1 in HeLa cell lysate
Exposure time is 120 secs for each time point
Images captured with ImageQuant™ LAS 4000 from GE Healthcare

Additional product information

Performance	Signal Duration
Detection Level: Mid-picogram

Two-fold dilution series of HeLa whole cell lysate from 5 μg to 0.078 μg of total protein on nitrocellulose membrane Primary antibody:Rabbit-anti Human HDAC-1 1:2000 Secondary antibody:Goat anti-rabbit IgG HRP (2mg/mL) 1:20000
Detection Level: Mid-femtogram

Two-fold dilution series of HeLa whole cell lysate from 5 μg to 0.078 μg of total protein on nitrocellulose membrane Primary antibody: Rabbit-anti Human HDAC-1 1:5000 Secondary antibody: Goat anti-rabbit IgG HRP (2mg/mL) 1:75000
Detection Level: Low-femtogram

Two-fold dilution series of HeLa whole cell lysate from 2.5 μg to 0.039 μg of total protein on nitrocellulose membrane Primary antibody: Rabbit-anti Human HDAC-1 1:10000 Secondary antibody: Goat anti-rabbit IgG HRP (2mg/mL) 1:200000

Specifications

Other specifications

Application	Western blotting
Brand	Western Lightning ONE
Detection Modality	Chemiluminescence
Sample Type	Chemiluminescent Substrate
Shipping Conditions	Shipped Ambient
Unit Size	125 mL