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  • Chemiluminescent Blotting Substrates
  • Western Lightning ONE Femto, Chemiluminescent Substrate (25 mL)

Western Lightning ONE Femto, Chemiluminescent Substrate (25 mL)

Western Lightning ONE Femto, Chemiluminescent Substrate (25 mL)
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Western Lightning ONE Femto, Chemiluminescent Substrate (25 mL)
Western Lightning ONE Femto, Chemiluminescent Substrate (25 mL)
Video Play Button
Western Lightning ONE Femto, Chemiluminescent Substrate (25 mL)
Western Lightning ONE Femto Chemiluminescent Substrate-premixed and ready to use for your convenience.
Western Lightning ONE Femto, Chemiluminescent Substrate (25 mL)
Western Lightning ONE Femto, Chemiluminescent Substrate (25 mL)
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A versatile, mid-level pre-mixed, one-component chemiluminescent horseradish peroxidase (HRP) substrate appropriate for a wide range of antibody dilutions, Western Lightning™ ONE Femto can detect mid-femtogram protein levels in western blots with a high signal intensity.

View product information
Feature Specification
Application ウェスタンブロッティング
Detection Modality 化学発光

A versatile, mid-level pre-mixed, one-component chemiluminescent horseradish peroxidase (HRP) substrate appropriate for a wide range of antibody dilutions, Western Lightning™ ONE Femto can detect mid-femtogram protein levels in western blots with a high signal intensity.

View product information
Product variants
Unit Size: 25 mL
Part #:
NEL140001EA
Unit Size: 250 mL
Part #:
NEL141001EA
Unit Size: 2 x 250 mL
Part #:
NEL142001EA
For research use only. Not for use in diagnostic procedures.
Request more information
Western Lightning ONE Femto, Chemiluminescent Substrate (25 mL)
Western Lightning ONE Femto, Chemiluminescent Substrate (25 mL)
Video Play Button
Western Lightning ONE Femto, Chemiluminescent Substrate (25 mL)
Western Lightning ONE Femto, Chemiluminescent Substrate (25 mL)
Video Play Button
Western Lightning ONE Femto, Chemiluminescent Substrate (25 mL)
Western Lightning ONE Femto Chemiluminescent Substrate-premixed and ready to use for your convenience.
Western Lightning ONE Femto, Chemiluminescent Substrate (25 mL)
Western Lightning ONE Femto, Chemiluminescent Substrate (25 mL)
Western Lightning ONE Femto, Chemiluminescent Substrate (25 mL)
Video Play Button
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Product information

  • Overview
  • Additional product information
  • Specifications
  • Video gallery

Overview

Get accurate detection of both low and high abundance proteins in the same experient. Part of our premier line of premixed chemiluminescnet HRP substrates, Western Lightning ONE Femto offers the flexibility of mid-femtogram detection with excellent dynamic range. This substrate offers sensitive and consistent detection of proteins with no mixing or measuring parts required. Stored and ready-to-use at room temperature, the 12 month extended shelf life means you can buy and use Western Lightning ONE Femto with confidence. Available in 25 mL size.

Comparison of Western Lightning™ ONE Versus Major Competitors

Performance and Signal Duration

  • Western blotting detection of HDAC-1 in HeLa cell lysate
  • Exposure time is 120 secs for each time point
  • Images captured with ImageQuant™ LAS 4000 from GE Healthcare

Additional product information

Performance Signal Duration

Detection Level: Mid-picogram

wlo-pico-1
wlo-pico-web
  • Two-fold dilution series of HeLa whole cell lysate from 5 μg to 0.078 μg of total protein on nitrocellulose membrane
  • Primary antibody:Rabbit-anti Human HDAC-1 1:2000
  • Secondary antibody:Goat anti-rabbit IgG HRP (2mg/mL) 1:20000

Detection Level: Mid-femtogram

wlo-femto-web
wlo-femto-web
  • Two-fold dilution series of HeLa whole cell lysate from 5 μg to 0.078 μg of total protein on nitrocellulose membrane
  • Primary antibody: Rabbit-anti Human HDAC-1 1:5000
  • Secondary antibody: Goat anti-rabbit IgG HRP (2mg/mL) 1:75000

Detection Level: Low-femtogram

wlo-femto-ultra-web
wlo-femto-ultra-web
  • Two-fold dilution series of HeLa whole cell lysate from 2.5 μg to 0.039 μg of total protein on nitrocellulose membrane
  • Primary antibody:  Rabbit-anti Human HDAC-1 1:10000
  • Secondary antibody: Goat anti-rabbit IgG HRP (2mg/mL) 1:200000

 

Specifications

Application
Western blotting
Brand
Western Lightning ONE
Detection Modality
Chemiluminescence
Sample Type
Chemiluminescent Substrate
Shipping Conditions
Shipped Ambient
Unit Size
25 mL

Video gallery

Western Lightning ONE Femto, Chemiluminescent Substrate (25 mL)
Western Lightning ONE Femto, Chemiluminescent Substrate (25 mL)

Resources

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The power of Western Lightning ONE HRP chemiluminescent substrates

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Get the consistency you demand from your western blot experiments with pre-mixed, one...

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Application Note
Western blot validation of CRISPR-Cas9-induced functional knockout using synthetic sgRNA molecules

Any well-designed CRISPR experiment must include a method to assess the accuracy and reliability of predicted gene editing results...

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Brochure
Western blotting solutions

With applications in biotechnology, molecular biology, proteomics, and more, western blotting is the most widely used technique...

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