Positive control lysate for AlphaLISA™ SureFire® Ultra™ Multiplex Phospho/Total Insulin Receptor β Detection Kit.
| Feature | Specification |
|---|---|
| Application | 細胞シグナル伝達 |
| Protocol Time | 2h at RT |
Positive control lysate for AlphaLISA™ SureFire® Ultra™ Multiplex Phospho/Total Insulin Receptor β Detection Kit.
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The positive control lysate is prepared from CHO cells transfected with the insulin receptor (CHO-INSR cells), cultured to confluence in T175 flasks in 10% FBS containing medium, then treated with 2μg/mL Insulin and 0.1% BSA for 15 minutes and lysed in 4mL of 1X SureFire Ultra Lysis buffer.
| Application |
Cell Signaling
|
|---|---|
| Automation Compatible |
Yes
|
| Brand |
AlphaLISA SureFire Ultra Multiplex
|
| Cellular or Signaling Pathway |
MAPK / ERK signaling
|
| Detection Modality |
Alpha
|
| Lysis Buffer Compatibility |
Lysis Buffer
|
| Molecular Modification |
Phosphorylation
|
| Product Group |
Control Lysate
|
| Protocol Time |
2h at RT
|
| Shipping Conditions |
Shipped in Blue Ice
|
| Target |
IR-β (Insulin Receptor β)
|
| Target Class |
Phosphoproteins
|
| Target Species |
Human
|
| Technology |
Alpha
|
| Unit Size |
1 vial
|
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