The purification of small RNA species such as microRNA (miRNA) is challenging because of the low concentrations, small fragment sizes (19-22 nucleotides) and tendency to be found in protein complexes. Due to its essential role in homeostasis, miRNA is an important biomarker for cancer and metabolic disease research.
With only minimal hands-on time, the chemagic miRNA kits enable high throughput and efficient extraction of circulating cell-free small RNA from 200 µl or 1 ml inputs in 24-well or 96-well format. The chemagic miRNA kits obtained equivalent yields to manual methods, while analysis by quantitative PCR and Next Generation Sequencing revealed high diversity and reliable performance.
| Feature | Specification |
|---|---|
| Instrument Compatibility | chemagic Prepito instrument |
| Nucleic Acid Type | miRNA |
| Sample Type |
血漿 唾液 |
The purification of small RNA species such as microRNA (miRNA) is challenging because of the low concentrations, small fragment sizes (19-22 nucleotides) and tendency to be found in protein complexes. Due to its essential role in homeostasis, miRNA is an important biomarker for cancer and metabolic disease research.
With only minimal hands-on time, the chemagic miRNA kits enable high throughput and efficient extraction of circulating cell-free small RNA from 200 µl or 1 ml inputs in 24-well or 96-well format. The chemagic miRNA kits obtained equivalent yields to manual methods, while analysis by quantitative PCR and Next Generation Sequencing revealed high diversity and reliable performance.
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption, and disposal requirements under European REACH regulations (EC 1907/2006).
The images shown are for illustrative purposes only and do not represent the exact contents or configuration of the actual kit. This kit may contain hazardous materials requiring special handling procedures; please refer to the provided Safety Data Sheets and product documentation for complete information.
The Prepito miRNA 200 Kit provides efficient isolation of pure small RNA, including microRNAs, from various biological samples. Designed specifically for use with the chemagic Prepito instrument, the optimized chemistry effectively separates miRNAs from larger RNA species and contaminants, resulting in enriched small RNA fractions suitable for demanding downstream applications. The streamlined workflow processes up to 12 samples simultaneously with minimal hands-on time, making it suitable for research laboratories focused on miRNA expression analysis, biomarker discovery, and regulatory RNA studies. The purified miRNA has high integrity and purity, supporting reliable results in applications such as RT-qPCR, microarray analysis, and next-generation sequencing.
| Application |
DNA/RNA Isolation
RNA
|
|---|---|
| Brand |
Prepito
|
| Format |
Automated
|
| Instrument Compatibility |
chemagic Prepito instrument
|
| Nucleic Acid Type |
miRNA
|
| Regulatory Status |
RUO
|
| Research Areas |
Biobanking
Genomic Analysis
Oncology
|
| Sample Type |
Plasma
Saliva
|
| Sample Volume |
Up to 200 ml
|
| Shelf Life |
24 months
|
| Storage Conditions |
Room Temperature
|
| Technology |
M-PVA Magnetic Bead Technology
|
| Throughput |
Up to 12 samples per run
|
| Unit Size |
180 preps/kit
|
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