PhenoVue ROS-490, Total Oxidative Stress Indicator

Reactive oxygen species (ROS) are naturally produced by all living organisms and participate in cell signaling, gene expression and homeostasis. Increasing levels of ROS is associated with aging, as well as with various diseases such as diabetes, pulmonary disorders, inflammatory diseases, and cancer. ROS are also produced by exogenous sources such as UV or heat. Ultimately elevated ROS concentration, which is known as oxidative stress, results in impairment of membrane lipids, proteins, and nucleic acids.

ROS are reactive molecules which contain oxygen and encompass various entities such as superoxide anion (O₂^-), hydrogen peroxide (H₂O₂), hydroxyl radical (•OH), hypochlorous acid (HOCl), peroxynitrite anion (ONOO^-), and nitric oxide (NO).

PhenoVue ROS Total Oxidative Stress Indicators, encompassing ROS-490, ROS-570 and ROS-670, enable a myriad of applications as well as multiplexing capability to unravel ROS related biological effects.

PhenoVue ROS-490, Total Oxidative Stress Indicator is a nonfluorescent cell-permeable probe which emits bright green fluorescence upon reaction with hydroxyl radical, hydrogen peroxide and hypochlorite anion.

Typical working concentration: 1-10 µM

Equivalent number of microplates:

• 8-24 x 96-well microplates
• 8-24 x 384-well microplates
• 13-40 x 1536-well microplates