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  • 研究・開発
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  • Cellular Imaging & Analysis
  • Cellular Imaging Reagents
  • PhenoVue 蛍光試薬&キット​
  • PhenoVue Live/Dead Cell Viability Assay Kit

PhenoVue Live/Dead Cell Viability Assay Kit

PhenoVue Live/Dead Cell Viability Assay Kit
90% viability cells (Live cells), 0% viability cells (Fixed cells) and mixture of two cells (Live/Fixed=50/50) analyzed with PhenoVue Live/Dead Cell Viability Assay Kit
PhenoVue Live/Dead Cell Viability Assay Kit
PhenoVue Live/Dead Cell Viability Assay Kit
90% viability cells (Live cells), 0% viability cells (Fixed cells) and mixture of two cells (Live/Fixed=50/50) analyzed with PhenoVue Live/Dead Cell Viability Assay Kit
PhenoVue Live/Dead Cell Viability Assay Kit
90% viability cells (Live cells), 0% viability cells (Fixed cells) and mixture of two cells (Live/Fixed=50/50) analyzed with PhenoVue Live/Dead Cell Viability Assay Kit
PhenoVue Live/Dead Cell Viability Assay Kit
PhenoVue Live/Dead Cell Viability Assay Kit
90% viability cells (Live cells), 0% viability cells (Fixed cells) and mixture of two cells (Live/Fixed=50/50) analyzed with PhenoVue Live/Dead Cell Viability Assay Kit
PhenoVue Live/Dead Cell Viability Assay Kit
90% viability cells (Live cells), 0% viability cells (Fixed cells) and mixture of two cells (Live/Fixed=50/50) analyzed with PhenoVue Live/Dead Cell Viability Assay Kit

PhenoVue™ Live/Dead Cell Viability Assay Kit enables the simultaneous determination of live and dead cells and is part of Revvity’s portfolio of cellular imaging reagents. Calcein AM dye is a cell permeable fluorescent dye which generates a bright green, fluorescent signal, whereas Propidium Iodide dye enters damaged cell membranes of dead cells and exhibits a bright red fluorescence. This assay kit provides an easy-to-use method for measuring cell proliferation, cell viability, cytotoxicity and apoptosis.

View our extensive validation data in the Product Information Sheet within the Resources tab below.

View product information

PhenoVue™ Live/Dead Cell Viability Assay Kit enables the simultaneous determination of live and dead cells and is part of Revvity’s portfolio of cellular imaging reagents. Calcein AM dye is a cell permeable fluorescent dye which generates a bright green, fluorescent signal, whereas Propidium Iodide dye enters damaged cell membranes of dead cells and exhibits a bright red fluorescence. This assay kit provides an easy-to-use method for measuring cell proliferation, cell viability, cytotoxicity and apoptosis.

View our extensive validation data in the Product Information Sheet within the Resources tab below.

View product information
Product variant
Quantity: 1 kit
Part #:
PCVA11
For research use only. Not for use in diagnostic procedures.
Request more information
PhenoVue Live/Dead Cell Viability Assay Kit
90% viability cells (Live cells), 0% viability cells (Fixed cells) and mixture of two cells (Live/Fixed=50/50) analyzed with PhenoVue Live/Dead Cell Viability Assay Kit
PhenoVue Live/Dead Cell Viability Assay Kit
90% viability cells (Live cells), 0% viability cells (Fixed cells) and mixture of two cells (Live/Fixed=50/50) analyzed with PhenoVue Live/Dead Cell Viability Assay Kit
PhenoVue Live/Dead Cell Viability Assay Kit
PhenoVue Live/Dead Cell Viability Assay Kit
90% viability cells (Live cells), 0% viability cells (Fixed cells) and mixture of two cells (Live/Fixed=50/50) analyzed with PhenoVue Live/Dead Cell Viability Assay Kit
PhenoVue Live/Dead Cell Viability Assay Kit
90% viability cells (Live cells), 0% viability cells (Fixed cells) and mixture of two cells (Live/Fixed=50/50) analyzed with PhenoVue Live/Dead Cell Viability Assay Kit
PhenoVue Live/Dead Cell Viability Assay Kit
PhenoVue Live/Dead Cell Viability Assay Kit
PhenoVue Live/Dead Cell Viability Assay Kit
90% viability cells (Live cells), 0% viability cells (Fixed cells) and mixture of two cells (Live/Fixed=50/50) analyzed with PhenoVue Live/Dead Cell Viability Assay Kit
PhenoVue Live/Dead Cell Viability Assay Kit
90% viability cells (Live cells), 0% viability cells (Fixed cells) and mixture of two cells (Live/Fixed=50/50) analyzed with PhenoVue Live/Dead Cell Viability Assay Kit
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Product information

  • Overview
  • Specifications
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Overview

PhenoVue Live/Dead Cell Viability Assay Kit provides a two-color green / red fluorescence method which enables the simultaneous determination of live and dead cells using two different dyes. Calcein AM dye penetrates live cells where intracellular esterases hydrolyze the dye to generate a bright green, fluorescent signal. Propidium Iodide dye enters damaged cell membranes of dead cells and undergoes a 40-fold enhancement of fluorescence upon binding to nucleic acid, thereby producing a bright red fluorescence. This assay kit provides an easy-to-use method for measuring cell proliferation, cell viability, cytotoxicity and apoptosis.

Kit Components / Quantity:

  • Calcein-AM/ 2 vials - lyophilized
  • Propidium Iodide / 1 vial - liquid
  • DMSO / 1 vial – liquid
  • Assay Buffer / 1 bottle - liquid

Equivalent number of microplates:

  • 2 x 96-well microplates, or
  • 2 x 384-well microplates, or
  • 3 x 1536-well microplates

Specifications

Color
Dual Green/Red
Application
High Content Imaging
Microscopy
Assay Points
2 x 96-well microplates
Brand
PhenoVue™
Cell Function Indicator
Cell viability
Detection Modality
Fluorescence
Filter
FITC/TRITC
Quantity
1 kit
Sample Type
Live samples only
Shipping Conditions
Shipped in Dry Ice
Storage Conditions
-16 °C or below, protected from light
Type
Kit

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eBook
Guide to live-cell imaging
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PhenoVue Cellular Imaging Reagents Brochure
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PhenoVue Cellular Imaging Reagents Flyer

This flyer describes Revvity's PhenoVue cellular imaging reagents.

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Product Info
PhenoVue Live/Dead Cell Viability Assay Kit Product Sheet

This is a product information sheet for PhenoVue Live/Dead Cell Viability Assay Kit

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Flyer
PhenoVue reagents & imaging microplates product list

PhenoVue reagents & imaging microplates product list

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