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PhenoVue Human IgG Antibody Internalization Kit (Fluor 647, pH Sensitive) - 10 x 96-wells
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PhenoVue Human IgG Antibody Internalization Kit (Fluor 647, pH Sensitive) - 10 x 96-wells
Representative fluorescence images of BT-474 cells showing Trastuzumab internalization compared to an IgG1 isotype control at 1 hour and 24 hours, acquired with the Opera Phenix™ Plus High-Content Screening System using the PhenoVue™ Human IgG Antibody Internalization Kit (Fluor 647, pH-sensitive). Nuclei are shown in blue, lysosomes in green, and the pH-sensitive Fab–Trastuzumab complex in red.
The PhenoVue™ human IgG antibody internalization kit offers a ready-to-use, reproducible method for studying antibody internalization. The kit contains PhenoVue Fluor 647 pH-sensitive Fab anti-human IgG, which binds with high affinity to the Fc region of antibodies, whether unconjugated or conjugated (including ADCs). This fluorescent Fab remains weakly fluorescent at neutral extracellular pH (≥7), but its signal increases upon entry into acidic intracellular compartments such as early and late endosomes and lysosomes. This pH-responsive behavior enhances the signal-to-background ratio, supporting clear and reliable visualization of internalization events.
| Feature | Specification |
|---|---|
| Quantity | 1 |
| Unit Size | 10 x 96-wells |
The PhenoVue™ human IgG antibody internalization kit offers a ready-to-use, reproducible method for studying antibody internalization. The kit contains PhenoVue Fluor 647 pH-sensitive Fab anti-human IgG, which binds with high affinity to the Fc region of antibodies, whether unconjugated or conjugated (including ADCs). This fluorescent Fab remains weakly fluorescent at neutral extracellular pH (≥7), but its signal increases upon entry into acidic intracellular compartments such as early and late endosomes and lysosomes. This pH-responsive behavior enhances the signal-to-background ratio, supporting clear and reliable visualization of internalization events.
Product variants
Assay Points: 2 x 96ウェルマイクロプレート
Part #:
PHAINTDR14
Assay Points: 10 x 96-well microplates
Part #:
PHAINTDR15
For research use only. Not for use in diagnostic procedures.
PhenoVue Human IgG Antibody Internalization Kit (Fluor 647, pH Sensitive) - 10 x 96-wells
Representative fluorescence images of BT-474 cells showing Trastuzumab internalization compared to an IgG1 isotype control at 1 hour and 24 hours, acquired with the Opera Phenix™ Plus High-Content Screening System using the PhenoVue™ Human IgG Antibody Internalization Kit (Fluor 647, pH-sensitive). Nuclei are shown in blue, lysosomes in green, and the pH-sensitive Fab–Trastuzumab complex in red.
PhenoVue Human IgG Antibody Internalization Kit (Fluor 647, pH Sensitive) - 10 x 96-wells
Product information
Overview
The study of antibody internalization is important in the research and development of antibody-drug conjugates (ADCs), a rapidly expanding class of biopharmaceuticals that link a monoclonal antibody to a cytotoxic payload via a cleavable linker. Upon internalization and lysosomal processing, the payload is released, triggering cell death through mechanisms such as DNA damage or microtubule disruption. Assessment of antibody internalization is therefore critical for the design, optimization, and evaluation of ADCs and related biologics.
The PhenoVue human IgG antibody internalization kit, which includes PhenoVue 488 lysosomal stain and PhenoVue Hoechst 33342 nuclear stain, facilitates no-wash live-cell, time-lapse monitoring of pH sensitive Fab conjugate-antibody complex trafficking into lysosomes.
The probes included in the kit are compatible with standard cell culture conditions and exert minimal impact on cell viability and proliferation, providing reliable results even during extended live-cell imaging studies.
Specifications
| Form |
Liquid
|
|---|
| Application |
High Content Imaging
Microscopy
|
|---|---|
| Assay Points |
10 x 96-well microplates
|
| Brand |
PhenoVue™
|
| Detection Modality |
Fluorescence
|
| Product Group |
Kit
|
| Quantity |
1
|
| Sample Type |
Live cells only
|
| Shipping Conditions |
Shipped in Dry Ice
|
| Storage Conditions |
-16° C or below, protected from light
|
| Target Species |
Human
|
| Type |
Kit
|
| Unit Size |
10 x 96-wells
|
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PhenoVue Human IgG Antibody Internalization Kit (Fluor 647, pH Sensitive) - 10 x 96-wells
PhenoVue Human IgG Antibody Internalization Kit (Fluor 647, pH Sensitive) - 10 x 96-wells
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Application Note
Live-cell imaging of ADC internalization and trafficking
Internalization and trafficking to lysosomes are critical steps for the efficacy of antibody-based treatments, especially antibody...
Product Info
PhenoVue human IgG antibody internalization kit (Fluor 647, pH sensitive) product information sheet
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