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  • 研究・開発
  • 細胞分析
  • Cellular Imaging & Analysis
  • Cellular Imaging Reagents
  • PhenoVue Fluorescent Probes for Organelles & Subcellular Compartments
  • PhenoVue Fluor 647 - WGA

PhenoVue Fluor 647 - WGA

PhenoVue Fluor 647 - WGA
HeLa fixed (PFA) cells stained with PhenoVue Fluor 647 WGA (Plasma Membrane). Imaged on Operetta CLS.
HeLa cells stained with PhenoVue Fluor 647 - WGA View All
PhenoVue Fluor 647 - WGA
PhenoVue Fluor 647 - WGA
HeLa fixed (PFA) cells stained with PhenoVue Fluor 647 WGA (Plasma Membrane). Imaged on Operetta CLS.
HeLa cells stained with PhenoVue Fluor 647 - WGA
HeLa fixed (PFA) cells stained with PhenoVue Fluor 647 WGA (Plasma Membrane) + PhenoVue Hoechst 33342 Nuclear Stain (nucleus, blue). Imaged on Operetta CLS.
PhenoVue Fluor 647 - WGA
HeLa fixed (PFA) cells stained with PhenoVue Fluor 647 WGA (Plasma Membrane). Imaged on Operetta CLS.
HeLa cells stained with PhenoVue Fluor 647 - WGA
HeLa fixed (PFA) cells stained with PhenoVue Fluor 647 WGA (Plasma Membrane) + PhenoVue Hoechst 33342 Nuclear Stain (nucleus, blue). Imaged on Operetta CLS.
PhenoVue Fluor 647 - WGA
PhenoVue Fluor 647 - WGA
HeLa fixed (PFA) cells stained with PhenoVue Fluor 647 WGA (Plasma Membrane). Imaged on Operetta CLS.
HeLa cells stained with PhenoVue Fluor 647 - WGA
HeLa fixed (PFA) cells stained with PhenoVue Fluor 647 WGA (Plasma Membrane) + PhenoVue Hoechst 33342 Nuclear Stain (nucleus, blue). Imaged on Operetta CLS.
PhenoVue Fluor 647 - WGA
HeLa fixed (PFA) cells stained with PhenoVue Fluor 647 WGA (Plasma Membrane). Imaged on Operetta CLS.
HeLa cells stained with PhenoVue Fluor 647 - WGA
HeLa fixed (PFA) cells stained with PhenoVue Fluor 647 WGA (Plasma Membrane) + PhenoVue Hoechst 33342 Nuclear Stain (nucleus, blue). Imaged on Operetta CLS.

PhenoVue™ Fluor 647 - WGA is a fluorescent lectin which displays high affinity for sialic acid and N-acetylglucosamine residues of glycoproteins and glycolipids present at the cellular plasma membranes. It can be used for cellular membrane staining, particularly the Golgi apparatus.

PhenoVue Fluor 647 - WGA exhibits bright green fluorescence and is validated for use in imaging microscopy and high-content screening applications.

Part of Revvity's portfolio of cellular imaging reagents, PhenoVue Fluor 647 - WGA has a maximum excitation wavelength of 650 nm and a maximum emission wavelength of 670 nm.

View our extensive validation data in the Product Information Sheet within the Resources tab below.

View product information
Feature Specification
Color Red
Filter Cy5
Fluorophore PhenoVue™ Fluor 647
Organelle and Cell Compartment Golgi Apparatus

PhenoVue™ Fluor 647 - WGA is a fluorescent lectin which displays high affinity for sialic acid and N-acetylglucosamine residues of glycoproteins and glycolipids present at the cellular plasma membranes. It can be used for cellular membrane staining, particularly the Golgi apparatus.

PhenoVue Fluor 647 - WGA exhibits bright green fluorescence and is validated for use in imaging microscopy and high-content screening applications.

Part of Revvity's portfolio of cellular imaging reagents, PhenoVue Fluor 647 - WGA has a maximum excitation wavelength of 650 nm and a maximum emission wavelength of 670 nm.

View our extensive validation data in the Product Information Sheet within the Resources tab below.

View product information
Product variant
Quantity: 5 x 1 mg
Part #:
CP16471
For research use only. Not for use in diagnostic procedures.
Request more information
PhenoVue Fluor 647 - WGA
HeLa fixed (PFA) cells stained with PhenoVue Fluor 647 WGA (Plasma Membrane). Imaged on Operetta CLS.
HeLa cells stained with PhenoVue Fluor 647 - WGA
HeLa fixed (PFA) cells stained with PhenoVue Fluor 647 WGA (Plasma Membrane) + PhenoVue Hoechst 33342 Nuclear Stain (nucleus, blue). Imaged on Operetta CLS.
PhenoVue Fluor 647 - WGA
HeLa fixed (PFA) cells stained with PhenoVue Fluor 647 WGA (Plasma Membrane). Imaged on Operetta CLS.
HeLa cells stained with PhenoVue Fluor 647 - WGA
HeLa fixed (PFA) cells stained with PhenoVue Fluor 647 WGA (Plasma Membrane) + PhenoVue Hoechst 33342 Nuclear Stain (nucleus, blue). Imaged on Operetta CLS.
PhenoVue Fluor 647 - WGA
PhenoVue Fluor 647 - WGA
HeLa fixed (PFA) cells stained with PhenoVue Fluor 647 WGA (Plasma Membrane). Imaged on Operetta CLS.
HeLa cells stained with PhenoVue Fluor 647 - WGA
HeLa fixed (PFA) cells stained with PhenoVue Fluor 647 WGA (Plasma Membrane) + PhenoVue Hoechst 33342 Nuclear Stain (nucleus, blue). Imaged on Operetta CLS.
PhenoVue Fluor 647 - WGA
HeLa fixed (PFA) cells stained with PhenoVue Fluor 647 WGA (Plasma Membrane). Imaged on Operetta CLS.
HeLa cells stained with PhenoVue Fluor 647 - WGA
HeLa fixed (PFA) cells stained with PhenoVue Fluor 647 WGA (Plasma Membrane) + PhenoVue Hoechst 33342 Nuclear Stain (nucleus, blue). Imaged on Operetta CLS.
PhenoVue Fluor 647 - WGA
PhenoVue Fluor 647 - WGA
PhenoVue Fluor 647 - WGA
HeLa fixed (PFA) cells stained with PhenoVue Fluor 647 WGA (Plasma Membrane). Imaged on Operetta CLS.
HeLa cells stained with PhenoVue Fluor 647 - WGA
HeLa fixed (PFA) cells stained with PhenoVue Fluor 647 WGA (Plasma Membrane) + PhenoVue Hoechst 33342 Nuclear Stain (nucleus, blue). Imaged on Operetta CLS.
PhenoVue Fluor 647 - WGA
HeLa fixed (PFA) cells stained with PhenoVue Fluor 647 WGA (Plasma Membrane). Imaged on Operetta CLS.
HeLa cells stained with PhenoVue Fluor 647 - WGA
HeLa fixed (PFA) cells stained with PhenoVue Fluor 647 WGA (Plasma Membrane) + PhenoVue Hoechst 33342 Nuclear Stain (nucleus, blue). Imaged on Operetta CLS.
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Product information

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  • Specifications
  • Image gallery
  • Spectra viewer

Overview

WGA is a plant lectin that binds different carbohydrate motifs and displays high affinity for sialic acid and N-acetylglucosamine residues of glycoproteins and glycolipids present at the cellular plasma membranes.

Fluorescent WGA derivatives are commonly used for staining the cellular membranes of mammalian cells, particularly Golgi apparatus which is glycoprotein-enriched. In addition, fluorescent WGA derivatives are also routinely used for the staining of skeletal muscle cells and tissues, as well as in cardiac fibrosis.

PhenoVue Fluor 647 - WGA can be used to visualize cellular membranes in immunofluorescence, immunohistochemistry and flow cytometry, as well as high-content analysis and screening applications.

See product information sheet for more information on fixed-cell staining.

Recommended working concentration: 5 µg/mL (146 nM)

Equivalent number of microplates:

  • 36 - 100 x 96-well microplates
  • 30 - 100 x 384-well microplates
  • 55 - 160 x 1536-well microplates

Specifications

Color
Red
Form
Lyophilized
Maximum Emission Wavelength (Emmax)
670 nm
Maximum Excitation Wavelength (Exmax)
650 nm
Application
High Content Imaging
Microscopy
Brand
PhenoVue™
Detection Modality
Fluorescence
Filter
Cy5
Fluorophore
PhenoVue™ Fluor 647
Organelle and Cell Compartment
Golgi Apparatus
Quantity
5 x 1 mg
Sample Type
Live and fixed samples
Shipping Conditions
Shipped Ambient
Storage Conditions
2-8 °C, protected from light
Type
Individual reagent

Image gallery

PhenoVue Fluor 647 - WGA
HeLa fixed (PFA) cells stained with PhenoVue Fluor 647 WGA (Plasma Membrane). Imaged on Operetta CLS.
HeLa cells stained with PhenoVue Fluor 647 - WGA
HeLa fixed (PFA) cells stained with PhenoVue Fluor 647 WGA (Plasma Membrane) + PhenoVue Hoechst 33342 Nuclear Stain (nucleus, blue). Imaged on Operetta CLS.
PhenoVue Fluor 647 - WGA
HeLa fixed (PFA) cells stained with PhenoVue Fluor 647 WGA (Plasma Membrane). Imaged on Operetta CLS.
HeLa cells stained with PhenoVue Fluor 647 - WGA
HeLa fixed (PFA) cells stained with PhenoVue Fluor 647 WGA (Plasma Membrane) + PhenoVue Hoechst 33342 Nuclear Stain (nucleus, blue). Imaged on Operetta CLS.
PhenoVue Fluor 647 - WGA
PhenoVue Fluor 647 - WGA
HeLa fixed (PFA) cells stained with PhenoVue Fluor 647 WGA (Plasma Membrane). Imaged on Operetta CLS.
HeLa cells stained with PhenoVue Fluor 647 - WGA
HeLa fixed (PFA) cells stained with PhenoVue Fluor 647 WGA (Plasma Membrane) + PhenoVue Hoechst 33342 Nuclear Stain (nucleus, blue). Imaged on Operetta CLS.
PhenoVue Fluor 647 - WGA
HeLa fixed (PFA) cells stained with PhenoVue Fluor 647 WGA (Plasma Membrane). Imaged on Operetta CLS.
HeLa cells stained with PhenoVue Fluor 647 - WGA
HeLa fixed (PFA) cells stained with PhenoVue Fluor 647 WGA (Plasma Membrane) + PhenoVue Hoechst 33342 Nuclear Stain (nucleus, blue). Imaged on Operetta CLS.
PhenoVue Fluor 647 - WGA
PhenoVue Fluor 647 - WGA
HeLa fixed (PFA) cells stained with PhenoVue Fluor 647 WGA (Plasma Membrane). Imaged on Operetta CLS.
HeLa cells stained with PhenoVue Fluor 647 - WGA
HeLa fixed (PFA) cells stained with PhenoVue Fluor 647 WGA (Plasma Membrane) + PhenoVue Hoechst 33342 Nuclear Stain (nucleus, blue). Imaged on Operetta CLS.
PhenoVue Fluor 647 - WGA
HeLa fixed (PFA) cells stained with PhenoVue Fluor 647 WGA (Plasma Membrane). Imaged on Operetta CLS.
HeLa cells stained with PhenoVue Fluor 647 - WGA
HeLa fixed (PFA) cells stained with PhenoVue Fluor 647 WGA (Plasma Membrane) + PhenoVue Hoechst 33342 Nuclear Stain (nucleus, blue). Imaged on Operetta CLS.

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Guide to live-cell imaging
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PhenoVue Cellular Imaging Reagents Brochure
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PhenoVue Cellular Imaging Reagents Flyer

This flyer describes Revvity's PhenoVue cellular imaging reagents.

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Product Info
PhenoVue Fluor - WGA Conjugates Product Information Sheet

Fluorescent WGA conjugates represent a method of choice for labelling the cellular membranes of mammalian cells, particularly...

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PhenoVue reagents & imaging microplates product list

PhenoVue reagents & imaging microplates product list

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