Feature | Specification |
---|---|
Color | Red |
Filter | Cy5 |
Fluorophore | PhenoVue™ Fluor 647 |
Organelle and Cell Compartment | Golgi Apparatus |
WGA is a plant lectin that binds different carbohydrate motifs and displays high affinity for sialic acid and N-acetylglucosamine residues of glycoproteins and glycolipids present at the cellular plasma membranes.
Fluorescent WGA derivatives are commonly used for staining the cellular membranes of mammalian cells, particularly Golgi apparatus which is glycoprotein-enriched. In addition, fluorescent WGA derivatives are also routinely used for the staining of skeletal muscle cells and tissues, as well as in cardiac fibrosis.
PhenoVue Fluor 647 - WGA can be used to visualize cellular membranes in immunofluorescence, immunohistochemistry and flow cytometry, as well as high-content analysis and screening applications.
See product information sheet for more information on fixed-cell staining.
Recommended working concentration: 5 µg/mL (146 nM)
Equivalent number of microplates:
Color |
Red
|
---|---|
Form |
Lyophilized
|
Maximum Emission Wavelength (Emmax) |
670 nm
|
Maximum Excitation Wavelength (Exmax) |
650 nm
|
Application |
High Content Imaging
Microscopy
|
---|---|
Brand |
PhenoVue™
|
Detection Modality |
Fluorescence
|
Filter |
Cy5
|
Fluorophore |
PhenoVue™ Fluor 647
|
Organelle and Cell Compartment |
Golgi Apparatus
|
Quantity |
5 x 1 mg
|
Sample Type |
Live and fixed samples
|
Shipping Conditions |
Shipped Ambient
|
Storage Conditions |
2-8 °C, protected from light
|
Type |
Individual reagent
|
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This flyer describes Revvity's PhenoVue cellular imaging reagents.
Fluorescent WGA conjugates represent a method of choice for labelling the cellular membranes of mammalian cells, particularly...
PhenoVue reagents & imaging microplates product list
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