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PhenoVue Fluor 647 Live Cell Actin Stain - 1x5nmol
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PhenoVue Fluor 647 Live Cell Actin Stain - 1x5nmol
PhenoVue Fluor 647 Live Cell Actin Stain - 1x5nmol
| Feature | Specification |
|---|---|
| Color | Red |
| Filter | Cy5 |
| Fluorophore | PhenoVue™ Fluor 647 |
| Organelle and Cell Compartment | Actin |
For research use only. Not for use in diagnostic procedures.
PhenoVue Fluor 647 Live Cell Actin Stain - 1x5nmol
PhenoVue Fluor 647 Live Cell Actin Stain - 1x5nmol
PhenoVue Fluor 647 Live Cell Actin Stain - 1x5nmol
Product information
Overview
PhenoVue™ Fluor 647 live cell actin stain is a no wash, cell permeable fluorogenic dye which specifically bind to actin filaments (F-actin) in live cells. Sensitive, rapid and photostable, PhenoVue Fluor 647 live cell actin stain exhibits far-red emission and can be multiplexed with blue, green and orange colors such as PhenoVue nuclear, lysosomal, mitochondrial or tubulin stains.
Like other actin stains derived from jasplakinolide, cytotoxicity can be observed with long exposure time (>24h) which can be significantly limited at concentrations comprised between 30 and 300nM while maintaining high brightness and image quality.
Depending on the cellular model, intracellular retention of PhenoVue Fluor 647 live cell actin stain can be further improved in the presence of efflux pump inhibitor such as PhenoVue Probenecid, Ready to Use Solution.
Typical working concentration: 100 nM
Equivalent number of microplates:
- 1 to 5 x 96-well microplates
- 1 to 5 x 384-well microplates
- 2 to 8 x 1536-well microplates
Specifications
| Color |
Red
|
|---|---|
| Form |
Solution in DMSO
|
| Maximum Emission Wavelength (Emmax) |
670 nm
|
| Maximum Excitation Wavelength (Exmax) |
650 nm
|
| Application |
High Content Imaging
Microscopy
|
|---|---|
| Brand |
PhenoVue™
|
| Detection Modality |
Fluorescence
|
| Filter |
Cy5
|
| Fluorophore |
PhenoVue™ Fluor 647
|
| Organelle and Cell Compartment |
Actin
|
| Quantity |
1 x 5 nmol
|
| Sample Type |
Live and fixed samples
|
| Shipping Conditions |
Shipped in Dry Ice
|
| Storage Conditions |
-16 °C or below, protected from light
|
| Type |
Individual reagent
|
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PhenoVue Fluor 647 Live Cell Actin Stain - 1x5nmol
PhenoVue Fluor 647 Live Cell Actin Stain - 1x5nmol
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PhenoVue Cellular Imaging Reagents Flyer
This flyer describes Revvity's PhenoVue cellular imaging reagents.
Product Info
PhenoVue Fluor Live Cell Actin Stain
This is a product information sheet for PhenoVue Fluor live cell actin stain. View validation data, product information, protocols...
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PhenoVue reagents & imaging microplates product list
PhenoVue reagents & imaging microplates product list
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